UniProt Accession

 PP4C_HUMAN; P60510; P33172 

Genbank Protein ID

 X70218; AF097996; BC001416 

Genbank Nucleotide ID

 CAA49753.1; AAC96318.1; AAH01416.1 

Protein Name

 Serine/threonine-protein phosphatase 4 catalytic subunit 

Protein Synonyms/Alias

 PP4C; Pp4; Protein phosphatase X; PP-X 

Gene Name

 PPP4C 

Gene Synonyms/Alias

 PPP4; PPX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
21	LRRCELIKESEVKAL	ubiquitination	[1]
26	LIKESEVKALCAKAR	ubiquitination	[1, 2, 3, 4]
31	EVKALCAKAREILVE	ubiquitination	[2, 3, 4]
63	HGQFYDLKELFRVGG	ubiquitination	[1, 2]
133	FYDECLRKYGSVTVW	ubiquitination	[1]
183	QIRTIDRKQEVPHDG	ubiquitination	[1]
299	ETRGIPSKKPVADYF	ubiquitination	[1, 5]
300	TRGIPSKKPVADYFL	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin. 

Sequence Annotation

 ACT_SITE 115 115 Proton donor (By similarity).
 METAL 54 54 Iron (By similarity).
 METAL 56 56 Iron (By similarity).
 METAL 82 82 Iron (By similarity).
 METAL 82 82 Manganese (By similarity).
 METAL 114 114 Manganese (By similarity).
 METAL 164 164 Manganese (By similarity).
 METAL 238 238 Manganese (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 307 307 Leucine methyl ester (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Iron; Manganese; Metal-binding; Methylation; Nucleus; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 307 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; NAS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0030289; C:protein phosphatase 4 complex; IEA:Compara.
 GO:0046872; F:metal ion binding; NAS:UniProtKB.
 GO:0004704; F:NF-kappaB-inducing kinase activity; NAS:UniProtKB.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0000226; P:microtubule cytoskeleton organization; NAS:UniProtKB.
 GO:0010569; P:regulation of double-strand break repair via homologous recombination; IMP:UniProtKB. 

Interpro

 IPR004843; Metallo_PEstase_dom.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 

Pfam

 PF00149; Metallophos 

SMART

 SM00156; PP2Ac 

PROSITE

 PS00125; SER_THR_PHOSPHATASE 

PRINTS

 PR00114; STPHPHTASE.