CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016110
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase SUPV3L1, mitochondrial 
Protein Synonyms/Alias
 Suppressor of var1 3-like protein 1; SUV3-like protein 1 
Gene Name
 Supv3l1 
Gene Synonyms/Alias
 Suv3l1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
99EVKKILDKFYKRQEIacetylation[1]
454SINEKGEKELEPITTacetylation[2, 3]
729EPLSPSDKELPLASRacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP- dependent multisubstrate helicase, able to unwind double stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA (By similarity). 
Sequence Annotation
 DOMAIN 194 334 Helicase ATP-binding.
 DOMAIN 353 521 Helicase C-terminal.
 NP_BIND 207 214 ATP (By similarity).
 REGION 650 779 Interaction with LAMTOR5, important for
 MOD_RES 99 99 N6-acetyllysine (By similarity).
 MOD_RES 725 725 Phosphoserine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; Mitochondrion; Mitochondrion nucleoid; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 779 AA 
Protein Sequence
MSLPRCTLLW ARLPAGRGAG PRAAPCSALR ALVGSFPGAS GRVPCLAASS SASGGSKAPN 60
TSLFVPLTVK PQGPSADGDV GAELTRPLDK NEVKKILDKF YKRQEIQKLS ADYGLDARLF 120
HQAFISFRNY IMQSHSLDVD IHIVLNDICF SAAHVDDLFP FFLRHAKQIF PVLECKDDLR 180
KISDLRIPPN WYPEARARQR KIIFHSGPTN SGKTYHAIQR YLSATSGVYC GPLKLLAHEI 240
FEKSNAAGVP CDLVTGEERL TVEPEGKQAT HVSCTVEMCN VATPYEVAVI DEIQMIRDPA 300
RGWAWTRALL GLCAEEVHLC GESAAINLVS ELLYTTGEEV EVQKYERLTP ISVLDHALES 360
LDNLQPGDCI VCFSKNDIYS VSRQIEIRGL ESAVIYGSLP PGTKLAQARK FNDPNDPCKI 420
LVATDAIGMG LNLSIRRIIF YSLIKPSINE KGEKELEPIT TSQALQIAGR AGRFSSHFKE 480
GQVTTMHRDD LALLKDILNR PVDPIQAAGL HPTAEQIEMF AYHLPETTLS NLIDIFVDFA 540
QVDGQYFVCN MDDFKFSAEL IQHIPLSLRV RYVFCTAPIN KKQPFVCSSL LQFARQYSRN 600
EPLTFAWLRR YIKWPLLPPK NIKDLMDLEA VHDVFDLYLW LSYRFIDMFP DSSLVRSLQK 660
ELDAIIQEGV HNITKLIKIS ESRKLLNLES LPSGDQSRLS GASKSPARRT RGTKSAGNKA 720
TEPLSPSDKE LPLASRLVQQ GLLTADMLRQ LQKEWLTQQP EHSREKVGTR RKKKDPDSD 779 
Gene Ontology
 GO:0045025; C:mitochondrial degradosome; ISS:UniProtKB.
 GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
 GO:0042645; C:mitochondrial nucleoid; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0034458; F:3'-5' RNA helicase activity; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; ISS:UniProtKB.
 GO:0003677; F:DNA binding; ISS:UniProtKB.
 GO:0003678; F:DNA helicase activity; ISS:UniProtKB.
 GO:0003725; F:double-stranded RNA binding; ISS:UniProtKB.
 GO:0070827; P:chromatin maintenance; ISS:UniProtKB.
 GO:0006310; P:DNA recombination; ISS:UniProtKB.
 GO:0000958; P:mitochondrial mRNA catabolic process; ISS:UniProtKB.
 GO:0035946; P:mitochondrial mRNA surveillance; ISS:UniProtKB.
 GO:0035945; P:mitochondrial ncRNA surveillance; ISS:UniProtKB.
 GO:0000965; P:mitochondrial RNA 3'-end processing; ISS:UniProtKB.
 GO:0070584; P:mitochondrion morphogenesis; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
 GO:0000962; P:positive regulation of mitochondrial RNA catabolic process; ISS:UniProtKB. 
Interpro
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR022192; SUV3_C. 
Pfam
 PF00271; Helicase_C
 PF12513; SUV3_C 
SMART
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS