CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011097
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tricalbin-3 
Protein Synonyms/Alias
  
Gene Name
 TCB3 
Gene Synonyms/Alias
 YML072C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
32VGGNLPQKVINQQERacetylation[1]
32VGGNLPQKVINQQERubiquitination[2]
971ENEQEMEKNPKEWEVubiquitination[2]
1005LSLNELIKQKSGILSacetylation[1]
1065IRDLNYSKLHFRVSKacetylation[1]
1529KINYGKDKNGQVPPVubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 May play a role in membrane trafficking. 
Sequence Annotation
 DOMAIN 478 580 C2 1.
 DOMAIN 785 881 C2 2.
 DOMAIN 1121 1218 C2 3.
 METAL 1150 1150 Calcium 1 (By similarity).
 METAL 1150 1150 Calcium 2 (By similarity).
 METAL 1156 1156 Calcium 1 (By similarity).
 METAL 1204 1204 Calcium 1 (By similarity).
 METAL 1204 1204 Calcium 2 (By similarity).
 METAL 1206 1206 Calcium 1 (By similarity).
 METAL 1206 1206 Calcium 2 (By similarity).
 METAL 1206 1206 Calcium 3 (By similarity).
 METAL 1212 1212 Calcium 2 (By similarity).
 METAL 1212 1212 Calcium 3 (By similarity).
 MOD_RES 67 67 Phosphoserine.
 MOD_RES 112 112 Phosphoserine.
 MOD_RES 1340 1340 Phosphoserine.
 MOD_RES 1342 1342 Phosphoserine.
 MOD_RES 1346 1346 Phosphoserine.
 MOD_RES 1350 1350 Phosphothreonine.
 MOD_RES 1354 1354 Phosphoserine.
 MOD_RES 1400 1400 Phosphoserine.  
Keyword
 Calcium; Calcium/phospholipid-binding; Cell membrane; Coiled coil; Complete proteome; Lipid-binding; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1545 AA 
Protein Sequence
MTGIKAQVHP PPDSTLFHEE EKKKVGGNLP QKVINQQERG SDHAPSGHHQ YHQLINHDAN 60
DTKTSNSVSD VSKGQKTADS NPEGKKQSSK DIFVASSAQK TNQLPGPNPQ GSIGAVPLEG 120
LRPKEFRSAP SRKPNKFDTS ITKPGVLDDL GKLDEKDIKE KFHLDSDDKL FPWQNVGEFH 180
ASGKGSPNTK MSRVIKAYIL ENFYNDWYCN IATVLGTCFF SWLFAYIGFS WWSMIFIFLG 240
TATVYNAEYT RFNRNIRDDL KRVTVEETLS DRVESTTWLN SFLSKFWVIY MPVLSQQVKD 300
NVNPQLAGVA PGYGIDALAI DEFTLGSKAP TIKGIKSYTK TGKNTVEMDW SFAFTPSDVS 360
DMTATEAREK INPKISLGVT LGKSFVSKTM PILVEDINVA GKMRIKVEFG KAFPNIKIVS 420
LQLLEPPLID FALKPIGGDT LGLDVMSFLP GLKSFVKNII NSNIGPMLFP PNHLDINVED 480
IMAAQSKEAI GVLAVTIASA DSLKGSDFIT NTVDPYIVMT TEDAVPGTDE EVRTSIKSNV 540
KNPRWNETKY LLLNTLEQKL NLKCFDFNDV RKDTVIGDLQ LDLADLLQNP VLDNQTAELR 600
SGTKSKGILH YSLHWFPVKE DKSEEKAVER AEAKAKGKKE DENEDTTEKE EDENEESSQT 660
DVGIAKITLQ KVKYLDTTSS MTGSLSPCAE LFIDGQKVKS YRTLRRINEP SWNETIEVLV 720
PSKSNSKFVL KIFDDRMNGK ALICEYSSSL DDIMTTLDTA QEFVKGSPQG DIYLDVSWKS 780
IEMTGAFAAA NSVSEPIGCI KLDVKDAIIK GDLSGVGDVD PYYTVSLNRR VLYKSIYHSD 840
TDHPIFDNST YVPIFSPNQI LTLEFHDYQK IGKDRFIGSV QIPTSNVFKK DPKSGKYVGN 900
NGKEEISKLK LKDHEHKVTE SIVNVSTTFI PINLVYSPEE LVNVEKLEKE LKEKKKKFEA 960
TQEENEQEME KNPKEWEVAE IEDPFDSDEK KINRKAKLSL NELIKQKSGI LSMQILEGTL 1020
SPSSAYLEIL ADDISYPVFI CMKPSQGKLN SEMANIFIRD LNYSKLHFRV SKKHIAKDSD 1080
DVISETSYST LKLLKQAYEE PMWLNFNGSK MKVRFLYTPT SVKLPSSESV EDTGYLNIKL 1140
ISGHGLKSAD RNGYSDPFVH IFVNDKKVFK SNIKKKTLDP VWNEDAKIPI LSRSKNQVIF 1200
NVLDWDRAGD NDDLGQASLD VSSLEVGKTY NWNLNLNTQG SIKLQGSFNP EYIKPSFDIV 1260
KGGITDKPMK IASGAAHATV GIAGTGIGAA TGVATGGLKK GGHLLKSLGG NPMKRSKSSN 1320
GNESNGAKKS SEKKSFDRRS PSNLNSTSVT PRASLDYDPS VPNTSYAPVQ SASPVVKPTD 1380
NTSSSSNKKD TPSSNSRGHS RASSFARTLA PHGTYNGFIT VVAAENVAKH VQIKISLTQG 1440
GRLKHIYKTK SQKANNDGVA VFDEECSFKA SPEANLVLGA ISHQRLSRDK DLGIAQINLG 1500
DPQIQQDGQI SVKLGDGHLI VKINYGKDKN GQVPPVPEVP QEYTQ 1545 
Gene Ontology
 GO:0005933; C:cellular bud; IDA:SGD.
 GO:0032541; C:cortical endoplasmic reticulum; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005544; F:calcium-dependent phospholipid binding; IEA:UniProtKB-KW.
 GO:0008289; F:lipid binding; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0090158; P:endoplasmic reticulum membrane organization; IGI:SGD.
 GO:0060304; P:regulation of phosphatidylinositol dephosphorylation; IGI:SGD. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR017147; Tricalbin. 
Pfam
 PF00168; C2 
SMART
 SM00239; C2 
PROSITE
 PS50004; C2 
PRINTS