| Tag | Content |
|---|
| CPLM ID | CPLM-011820 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pre-mRNA-splicing factor CLF1 |
Protein Synonyms/Alias | Crooked neck-like factor 1; PRP19-associated complex protein 77; Synthetic lethal with CDC40 protein 3 |
Gene Name | CLF1 |
Gene Synonyms/Alias | NTC77; SYF3; YLR117C; L2952 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 673 | DDKPKPSKFLELAKK | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly by promoting the functional integration of the U4/U6.U5 tri-snRNP particle into the U1-, U2-dependent pre-spliceosome. Also recruits PRP19 to the spliceosome, as a component of the NTC complex (or PRP19- associated complex). The association of the NTC complex to the spliceosome mediates conformational rearrangement or stabilizes the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Required for initiation of the DNA replication by binding the RNA replication origins, probably through its interaction with the origin recognition complex (ORC). |
Sequence Annotation | REPEAT 45 77 HAT 1.
REPEAT 79 111 HAT 2.
REPEAT 113 145 HAT 3.
REPEAT 147 178 HAT 4.
REPEAT 180 211 HAT 5.
REPEAT 213 247 HAT 6.
REPEAT 251 283 HAT 7.
REPEAT 300 332 HAT 8.
REPEAT 337 369 HAT 9.
REPEAT 383 416 HAT 10.
REPEAT 451 483 HAT 11.
REPEAT 525 557 HAT 12.
REPEAT 629 661 HAT 13. |
Keyword | Cell cycle; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 687 AA |
Protein Sequence | MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR 60
NRLDMGQWIR YAQFEIEQHD MRRARSIFER ALLVDSSFIP LWIRYIDAEL KVKCINHARN 120
LMNRAISTLP RVDKLWYKYL IVEESLNNVE IVRSLYTKWC SLEPGVNAWN SFVDFEIRQK 180
NWNGVREIYS KYVMAHPQMQ TWLKWVRFEN RHGNTEFTRS VYSLAIDTVA NLQNLQIWSD 240
MEVAKLVNSF AHWEAAQQEY ERSSALYQIA IEKWPSNQLL KAGLLDFEKQ FGDINSIEET 300
ISYKRKMEYE TILSNNAYDY DTWWLYLDLI SESFPKQIMQ TFEKAIVDSR PKELSKNVQW 360
KRYIYLWMRY ICYVELELEN SLLEEELFQR LIDDIIPHKH FTFSKIWLMY AKFLIRHDDV 420
PKARKILGKA IGLCPKAKTF KGYIELEVKL KEFDRVRKIY EKFIEFQPSD LQIWSQYGEL 480
EENLGDWDRV RGIYTIALDE NSDFLTKEAK IVLLQKYITF ETESQEFEKA RKLYRRYLEL 540
NQYSPQSWIE FAMYQTSTPT EQQLLDLAKL QSENVDEDIE FEITDENKLE ARKVFEEAIV 600
FFKEKDDKQG RLSILEALKD YEETYGTELD QETVKKRFPK VIKKVRLQNG VEEEFVDYIF 660
PDDIDDDKPK PSKFLELAKK WKQEQAL 687 |
Gene Ontology | GO:0000785; C:chromatin; IDA:SGD. GO:0000974; C:Prp19 complex; IDA:SGD. GO:0071006; C:U2-type catalytic step 1 spliceosome; IDA:SGD. GO:0071007; C:U2-type catalytic step 2 spliceosome; IDA:SGD. GO:0071008; C:U2-type post-mRNA release spliceosomal complex; IDA:SGD. GO:0071004; C:U2-type prespliceosome; IDA:SGD. GO:0003682; F:chromatin binding; IDA:SGD. GO:0003688; F:DNA replication origin binding; IDA:SGD. GO:0007049; P:cell cycle; IEA:UniProtKB-KW. GO:0000354; P:cis assembly of pre-catalytic spliceosome; IMP:SGD. GO:0006270; P:DNA replication initiation; IMP:SGD. |
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