UniProt Accession

 DHOM_YEAST; P31116; D6VWV8 

Genbank Protein ID

 X64457; Z49639; BK006943 

Genbank Nucleotide ID

 CAA45787.1; CAA89671.1; DAA08924.1 

Protein Name

 Homoserine dehydrogenase 

Protein Synonyms/Alias

 HDH 

Gene Name

 HOM6 

Gene Synonyms/Alias

 YJR139C; J2132 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
48	AERSLISKDFSPLNV	acetylation	[1]
69	ALAASTTKTLPLDDL	acetylation	[1]
69	ALAASTTKTLPLDDL	ubiquitination	[2]
81	DDLIAHLKTSPKPVI	acetylation	[1]
117	ISIATPNKKAFSSDL	ubiquitination	[2]
118	SIATPNKKAFSSDLA	ubiquitination	[2]
199	VKFSDVVKVAKKLGY	acetylation	[1]
203	DVVKVAKKLGYTEPD	ubiquitination	[2]
223	NGLDVARKVTIVGRI	ubiquitination	[2]
255	PKPLESVKSADEFLE	acetylation	[1]
263	SADEFLEKLSDYDKD	acetylation	[1]
269	EKLSDYDKDLTQLKK	acetylation	[1]
283	KEAATENKVLRFIGK	acetylation	[1]
283	KEAATENKVLRFIGK	ubiquitination	[2]
290	KVLRFIGKVDVATKS	ubiquitination	[2, 3]
296	GKVDVATKSVSVGIE	ubiquitination	[2]
304	SVSVGIEKYDYSHPF	acetylation	[1]
304	SVSVGIEKYDYSHPF	ubiquitination	[2]
315	SHPFASLKGSDNVIS	acetylation	[1]
324	SDNVISIKTKRYTNP	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 18 NADP.
 ACT_SITE 223 223 Proton donor (Potential).
 BINDING 93 93 NADP.
 BINDING 117 117 NADP.
 BINDING 208 208 Substrate.  

Keyword

 3D-structure; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Isoleucine biosynthesis; Methionine biosynthesis; NADP; Oxidoreductase; Reference proteome; Threonine biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 359 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004412; F:homoserine dehydrogenase activity; IDA:SGD.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0009090; P:homoserine biosynthetic process; IMP:SGD.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009086; P:methionine biosynthetic process; IMP:SGD.
 GO:0009088; P:threonine biosynthetic process; IMP:SGD. 

Interpro

 IPR005106; Asp/hSer_DH_NAD-bd.
 IPR001342; HDH_cat.
 IPR019811; HDH_CS.
 IPR022697; HDH_short.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00742; Homoserine_dh
 PF03447; NAD_binding_3 

SMART

  

PROSITE

 PS01042; HOMOSER_DHGENASE 

PRINTS