UniProt Accession

 RANG_HUMAN; P43487 

Genbank Protein ID

 X83617; D38076; CR456556 

Genbank Nucleotide ID

 CAA58592.1; BAA07269.1; CAG30442.1 

Protein Name

 Ran-specific GTPase-activating protein 

Protein Synonyms/Alias

 Ran-binding protein 1; RanBP1 

Gene Name

 RANBP1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	EDEEELFKMRAKLFR	ubiquitination	[1]
68	ENDLPEWKERGTGDV	ubiquitination	[1, 2, 3, 4, 5]
76	ERGTGDVKLLKHKEK	ubiquitination	[1, 2, 4, 5]
111	ITPMMELKPNAGSDR	acetylation	[6]
111	ITPMMELKPNAGSDR	ubiquitination	[1, 3]
134	DFADECPKPELLAIR	acetylation	[6]
134	DFADECPKPELLAIR	ubiquitination	[1, 2, 5]
150	LNAENAQKFKTKFEE	acetylation	[7]
150	LNAENAQKFKTKFEE	ubiquitination	[1, 2, 3, 4, 5]
152	AENAQKFKTKFEECR	acetylation	[6]
154	NAQKFKTKFEECRKE	ubiquitination	[1]
183	HAEKVAEKLEALSVK	acetylation	[7]
183	HAEKVAEKLEALSVK	ubiquitination	[1, 2, 3, 4, 5, 8]
190	KLEALSVKEETKEDA	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

 Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. 

Sequence Annotation

 DOMAIN 26 164 RanBD1.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 13 13 Phosphothreonine.
 MOD_RES 18 18 Phosphothreonine.
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 150 150 N6-acetyllysine.
 MOD_RES 183 183 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; TAS:UniProtKB.
 GO:0005635; C:nuclear envelope; TAS:Reactome.
 GO:0005092; F:GDP-dissociation inhibitor activity; TAS:ProtInc.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0008536; F:Ran GTPase binding; TAS:ProtInc.
 GO:0046907; P:intracellular transport; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0046604; P:positive regulation of mitotic centrosome separation; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:UniProtKB.
 GO:0007051; P:spindle organization; IEA:Compara.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR011993; PH_like_dom.
 IPR000156; Ran_bind_dom. 

Pfam

 PF00638; Ran_BP1 

SMART

 SM00160; RanBD 

PROSITE

 PS50196; RANBD1 

PRINTS