UniProt Accession

 TRMB_ECOLI; P0A8I5; P32049; P58089; Q2M9N3 

Genbank Protein ID

 M59471; U28377; U00096; AP009048 

Genbank Nucleotide ID

 AAA72956.1; AAA69127.1; AAC75997.1; BAE77023.1 

Protein Name

 tRNA (guanine-N(7)-)-methyltransferase 

Protein Synonyms/Alias

 tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase 

Gene Name

 trmB 

Gene Synonyms/Alias

 trmI; yggH; b2960; JW2927 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
128	DAVEVLHKMIPDNSL	acetylation	[1]
148	FFPDPWHKARHNKRR	acetylation	[1]
166	VPFAELVKSKLQLGG	acetylation	[1]
218	PASRPVTKFEQRGHR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. 

Sequence Annotation

 REGION 150 155 Interaction with RNA (Potential).
 REGION 217 220 Substrate binding (Potential).
 BINDING 69 69 S-adenosyl-L-methionine (By similarity).
 BINDING 94 94 S-adenosyl-L-methionine (By similarity).
 BINDING 121 121 S-adenosyl-L-methionine (By similarity).
 BINDING 144 144 S-adenosyl-L-methionine (By similarity).
 BINDING 148 148 Substrate (By similarity).
 BINDING 180 180 Substrate (Potential).  

Keyword

 3D-structure; Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 239 AA 

Protein Sequence

Gene Ontology

 GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
 GO:0036265; P:RNA (guanine-N7)-methylation; IEA:GOC. 

Interpro

 IPR003358; tRNA_(Gua-N-7)_MeTrfase. 

Pfam

 PF02390; Methyltransf_4 

SMART

  

PROSITE

  

PRINTS