CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017566
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spermatogenesis-associated protein 5 
Protein Synonyms/Alias
 ATPase family protein 2 homolog; Spermatogenesis-associated factor protein 
Gene Name
 SPATA5 
Gene Synonyms/Alias
 AFG2; SPAF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
110MAGFPGGKVGLSEMAubiquitination[1]
189TFYGRPYKLQVLRVKubiquitination[2]
196KLQVLRVKGADGMILubiquitination[1, 3]
254TSRSTPYKPIDDRITubiquitination[1, 4]
263IDDRITNKASDVLLDubiquitination[5]
311TEEERLLKFSIGAKCubiquitination[2]
339VNFTEIDKNSKEQDNubiquitination[1]
342TEIDKNSKEQDNQFKubiquitination[1]
363GGLSSQLKAIREIIEubiquitination[1]
374EIIELPLKQPELFKSubiquitination[1, 3, 4]
380LKQPELFKSYGIPAPubiquitination[1]
400YGPPGTGKTMIARAVubiquitination[1]
434FYGETEAKLRQIFAEubiquitination[1]
461ELDALCPKREGAQNEubiquitination[1]
471GAQNEVEKRVVASLLacetylation[4]
535DRLDILQKLLRRVPHubiquitination[1]
600GLVKITLKDFLQAMNubiquitination[1]
636IGGLESIKLKLEQAVubiquitination[5]
638GLESIKLKLEQAVEWubiquitination[1, 5]
648QAVEWPLKHPESFIRubiquitination[1]
674YGPPGCSKTMIAKALubiquitination[1]
679CSKTMIAKALANESGubiquitination[1]
693GLNFLAIKGPELMNKubiquitination[1, 3, 5]
700KGPELMNKYVGESERubiquitination[1, 3, 5]
887FYEDYQEKSGLHTL*ubiquitination[1, 6, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 May be involved in morphological and functional mitochondrial transformations during spermatogenesis (By similarity). 
Sequence Annotation
 NP_BIND 394 401 ATP 1 (Potential).
 NP_BIND 668 675 ATP 2 (Potential).
 MOD_RES 272 272 Phosphothreonine.
 MOD_RES 274 274 Phosphoserine.
 MOD_RES 279 279 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Mitochondrion; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 893 AA 
Protein Sequence
MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN LLEKVDDKIP 60
KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA WPMAGFPGGK VGLSEMAQKN 120
VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD KDMEINEEEL TGCILRKLDG KIVLPGNFLY 180
CTFYGRPYKL QVLRVKGADG MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL 240
EDTQIPTSRS TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 300
EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV TYDMIGGLSS 360
QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK TMIARAVANE VGAYVSVING 420
PEIISKFYGE TEAKLRQIFA EATLRHPSII FIDELDALCP KREGAQNEVE KRVVASLLTL 480
MDGIGSEVSE GQVLVLGATN RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV 540
PHLLTEAELL QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 600
DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP ESFIRMGIQP 660
PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK YVGESERAVR ETFRKARAVA 720
PSIIFFDELD ALAVERGSSL GAGNVADRVL AQLLTEMDGI EQLKDVTILA ATNRPDRIDK 780
ALMRPGRIDR IIYVPLPDAA TRREIFKLQF HSMPVSNEVD LDELILQTDA YSGAEIVAVC 840
REAALLALEE DIQANLIMKR HFTQALSTVT PRIPESLRRF YEDYQEKSGL HTL 893 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR009010; Asp_de-COase-like_dom.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR003338; CDC4_N-term_subdom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00004; AAA 
SMART
 SM00382; AAA
 SM01073; CDC48_N 
PROSITE
 PS00674; AAA 
PRINTS