UniProt Accession

 DET1_YEAST; Q99288; D6VS38; P87337; Q05314; Q7LHX4 

Genbank Protein ID

 Z49209; Z74347; Z74348; X84162; BK006938 

Genbank Nucleotide ID

 CAA89081.1; CAA98868.1; CAA98870.1; CAA58968.1; DAA11898.1 

Protein Name

 Broad-range acid phosphatase DET1 

Protein Synonyms/Alias

 Decreased ergosterol transport protein 1 

Gene Name

 DET1 

Gene Synonyms/Alias

 YDR051C; D4202 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
110	YTRLSREKDTNIVFY	acetylation	[1]
186	EDNICEGKPGKCYLQ	ubiquitination	[2]
324	VLRTVLPKWTDCEGD	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Metal-independent, broad-range acid phosphatase. Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane. 

Sequence Annotation

 REGION 44 45 Substrate binding (By similarity).
 REGION 168 171 Substrate binding (By similarity).
 REGION 195 205 Substrate bindingy (By similarity).
 ACT_SITE 32 32 Tele-phosphohistidine intermediate (By
 ACT_SITE 274 274 By similarity.
 BINDING 38 38 Substrate (By similarity).
 BINDING 108 108 Substrate (By similarity).
 MOD_RES 248 248 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Lipid transport; Nucleus; Phosphoprotein; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003993; F:acid phosphatase activity; IDA:SGD.
 GO:0032366; P:intracellular sterol transport; IMP:SGD. 

Interpro

 IPR013078; His_Pase_superF_clade-1.
 IPR001345; PG/BPGM_mutase_AS. 

Pfam

 PF00300; His_Phos_1 

SMART

 SM00855; PGAM 

PROSITE

 PS00175; PG_MUTASE 

PRINTS