CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029712
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HLA-B associated transcript 3, isoform CRA_a 
Protein Synonyms/Alias
 Large proline-rich protein BAG6 
Gene Name
 BAG6 
Gene Synonyms/Alias
 BAT3; hCG_2005629 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22DSLEVLVKTLDSQTRubiquitination[1]
40VGAQMNVKEFKEHIAubiquitination[1, 2, 3, 4]
56SVSIPSEKQRLIYQGubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
70GRVLQDDKKLQEYNVubiquitination[8]
71RVLQDDKKLQEYNVGubiquitination[4]
80QEYNVGGKVIHLVERubiquitination[1, 2, 3, 4, 5, 7, 8, 9]
1027IQSQRKVKPQPPLSDubiquitination[2, 4]
1043YLSGMPAKRRKTMQGubiquitination[1, 4, 8]
1046GMPAKRRKTMQGEGPubiquitination[1, 2, 8]
1066EAVSRAAKAAGARPLubiquitination[4, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1126 AA 
Protein Sequence
MEPNDSTSTA VEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 60
YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTHLPSGASS GTGSASATHG GGSPPGTRGP 120
GASVHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 180
SRMECRGGPQ PQHSQPPPQP PAVTPEPVAL SSQTSEPVES EAPPREPMEA EEVEERAPAQ 240
NPELTPGPAP AGPTPAPETN APNHPSPAEY VEVLQELQRL ESRLQPFLQR YYEVLGAAAT 300
TDYNNNHEGR EEDQRLINLV GESLRLLGNT FVALSDLRCN LACTPPRHLH VVRPMSHYTT 360
PMVLQQAAIP IQINVGTTVT MTGNGTRPPP TPNAEAPPPG PGQASSVAPS STNVESSAEG 420
APPPGPAPPP ATSHPRVIRI SHQSVEPVVM MHMNIQDSGT QPGGVPSAPT GPLGPPGHGQ 480
TLGQQVPGFP TAPTRVVIAR PTPPQARPSH PGGPPVSGTL QGAGLGTNAS LAQMVSGLVG 540
QLLMQPVLVA QGTPGMAPPP APATASASAG TTNTATTAGP APGGPAQPPP TPQPSMADLQ 600
FSQLLGNLLG PAGPGAGGPG VASPTITVAM PGVPAFLQGM TDFLQATQTA PPPPPPPPPP 660
PPAPEQQTMP PPGSPSGGAG SPGGLGLESL SPEFFTSVVQ GVLSSLLGSL GARAGSSESI 720
AAFIQRLSGS SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR 780
SFFHQHYLGG QEPTPSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR TNLEFLQEQF 840
NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG QQMELAAVIN GRIRRMSRGV 900
NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI LRYVRRVGDP PQPLPEEPME VQGAERASPE 960
PQRENASPAP GTTAEEAMSR GPPPAPEGGS RDEQDGASAE TEPWAAAVPP EWVPIIQQDI 1020
QSQRKVKPQP PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS 1080
RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAQR AFADDP 1126 
Gene Ontology
  
Interpro
 IPR021925; DUF3538.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12057; DUF3538
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS