CPLM-017794

Genbank Nucleotide ID

Cytosolic 10-formyltetrahydrofolate dehydrogenase

Protein Synonyms/Alias

10-FTHFDH; FDH; Aldehyde dehydrogenase family 1 member L1

Mus musculus (Mouse)

Position	Peptide	Type	References
21	EVYCQLRKEGHEVVG	ubiquitination	[1]
35	GVFTIPDKDGKADPL	acetylation	[2, 3, 4]
38	TIPDKDGKADPLGLE	acetylation	[5]
38	TIPDKDGKADPLGLE	succinylation	[5]
38	TIPDKDGKADPLGLE	ubiquitination	[1]
48	PLGLEAEKDGVPVFK	acetylation	[2]
48	PLGLEAEKDGVPVFK	ubiquitination	[1]
55	KDGVPVFKFPRWRAR	ubiquitination	[1]
128	WTLIHGDKKGGFTIF	acetylation	[3]
128	WTLIHGDKKGGFTIF	ubiquitination	[1]
205	ATYEGIQKKETAMIN	acetylation	[3]
205	ATYEGIQKKETAMIN	ubiquitination	[1]
206	TYEGIQKKETAMINW	ubiquitination	[1]
229	NWIRGNDKVPGAWTE	acetylation	[2, 3, 4]
229	NWIRGNDKVPGAWTE	ubiquitination	[1]
290	DDRMLLVKNIQLEDG	ubiquitination	[1]
298	NIQLEDGKMMPASQF	ubiquitination	[1]
349	EDSTDFFKSGAASVD	ubiquitination	[1]
394	FIQLLVRKLRGEDGE	ubiquitination	[1]
411	CVINYVEKAVKKLTL	acetylation	[2]
411	CVINYVEKAVKKLTL	ubiquitination	[1]
415	YVEKAVKKLTLQMPY	ubiquitination	[1]
463	AQVSDVDKAVAAAKE	acetylation	[2]
469	DKAVAAAKEAFENGL	ubiquitination	[1]
479	FENGLWGKINARDRG	acetylation	[2, 3, 6]
479	FENGLWGKINARDRG	ubiquitination	[1]
597	AGNTVVIKPAQVTPL	ubiquitination	[1]
608	VTPLTALKFAELTLK	ubiquitination	[1]
615	KFAELTLKAGIPKGV	ubiquitination	[1]
620	TLKAGIPKGVVNILP	ubiquitination	[1]
645	SDHPDVRKIGFTGST	ubiquitination	[1]
656	TGSTEVGKHIMKSCA	ubiquitination	[1]
660	EVGKHIMKSCALSNV	acetylation	[2, 3, 6]
660	EVGKHIMKSCALSNV	ubiquitination	[1]
668	SCALSNVKKVSLELG	ubiquitination	[1]
669	CALSNVKKVSLELGG	ubiquitination	[1]
677	VSLELGGKSPLIIFA	ubiquitination	[1]
703	MSSVFFNKGENCIAA	acetylation	[2]
703	MSSVFFNKGENCIAA	ubiquitination	[1]
726	IHDQFVQKVVEEVGK	ubiquitination	[1]
757	NHEAHLRKLVEYCQR	ubiquitination	[1]
767	EYCQRGVKEGATLVC	acetylation	[2, 5]
767	EYCQRGVKEGATLVC	succinylation	[5]
767	EYCQRGVKEGATLVC	ubiquitination	[1]
845	VFTRDINKALYVSDK	ubiquitination	[1]
852	KALYVSDKLQAGTVF	ubiquitination	[1]
865	VFVNTYNKTDVAAPF	acetylation	[2]
865	VFVNTYNKTDVAAPF	ubiquitination	[1]
876	AAPFGGFKQSGFGKD	acetylation	[2]
876	AAPFGGFKQSGFGKD	ubiquitination	[1]
882	FKQSGFGKDLGEAAL	acetylation	[2, 3, 4, 6]
882	FKQSGFGKDLGEAAL	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]

Functional Description

DOMAIN 323 392 Acyl carrier.
NP_BIND 571 573 NADP (By similarity).
NP_BIND 597 600 NADP (By similarity).
NP_BIND 630 635 NADP (By similarity).
NP_BIND 650 651 NADP (By similarity).
NP_BIND 804 806 NADP (By similarity).
REGION 1 203 GART.
REGION 88 90 Substrate binding (By similarity).
REGION 417 902 Aldehyde dehydrogenase.
ACT_SITE 106 106 Proton donor (By similarity).
ACT_SITE 673 673 Proton acceptor (By similarity).
ACT_SITE 707 707 Proton donor (By similarity).
BINDING 142 142 Substrate (By similarity).
BINDING 757 757 NADP (By similarity).
MOD_RES 354 354 O-(pantetheine 4'-phosphoryl)serine;
MOD_RES 354 354 Phosphoserine; alternate (By similarity).

Complete proteome; Cytoplasm; NADP; One-carbon metabolism; Oxidoreductase; Phosphopantetheine; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0016155; F:formyltetrahydrofolate dehydrogenase activity; IEA:EC.
GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
GO:0008168; F:methyltransferase activity; IEA:InterPro.
GO:0016620; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; IEA:InterPro.
GO:0009258; P:10-formyltetrahydrofolate catabolic process; IEA:InterPro.
GO:0009058; P:biosynthetic process; IEA:InterPro.
GO:0032259; P:methylation; IEA:GOC.
GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.

IPR011407; 10_FTHF_DH.
IPR009081; Acyl_carrier_prot-like.
IPR016161; Ald_DH/histidinol_DH.
IPR016163; Ald_DH_C.
IPR016160; Ald_DH_CS.
IPR016162; Ald_DH_N.
IPR015590; Aldehyde_DH_dom.
IPR005793; Formyl_trans_C.
IPR002376; Formyl_transf_N.
IPR011034; Formyl_transferase_C-like.
IPR001555; GART_AS.

PF00171; Aldedh
PF02911; Formyl_trans_C
PF00551; Formyl_trans_N
PF00550; PP-binding

PS50075; ACP_DOMAIN
PS00070; ALDEHYDE_DEHYDR_CYS
PS00687; ALDEHYDE_DEHYDR_GLU
PS00373; GART
PS00012; PHOSPHOPANTETHEINE