UniProt Accession

 IF4E_YEAST; P07260; D6W1T0 

Genbank Protein ID

 M15436; M21620; M29251; X84036; Z74881; AY692936; BK006948 

Genbank Nucleotide ID

 AAA34587.1; AAA34480.1; AAA34588.1; CAA58854.1; CAA99160.1; AAT92955.1; DAA10646.1 

Protein Name

 Eukaryotic translation initiation factor 4E 

Protein Synonyms/Alias

 eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein 

Gene Name

 CDC33 

Gene Synonyms/Alias

 TIF45; YOL139C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
9	SVEEVSKKFEENVSV	acetylation	[1]
9	SVEEVSKKFEENVSV	ubiquitination	[2]
36	DSAHFDVKHPLNTKW	acetylation	[1]
111	WEDEANAKGGKWSFQ	ubiquitination	[2, 3]
114	EANAKGGKWSFQLRG	acetylation	[1]
114	EANAKGGKWSFQLRG	ubiquitination	[2, 3]
162	SIRKGGNKFALWTKS	acetylation	[1]
162	SIRKGGNKFALWTKS	ubiquitination	[2]
172	LWTKSEDKEPLLRIG	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. 

Sequence Annotation

 MOD_RES 2 2 Phosphoserine; by CK2.
 MOD_RES 15 15 Phosphoserine; by CK2.
 MOD_RES 22 22 Phosphothreonine.
 MOD_RES 28 28 Phosphoserine.
 MOD_RES 30 30 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 213 AA 

Protein Sequence

Gene Ontology

 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005840; C:ribosome; TAS:SGD.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; ISS:SGD.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:SGD.
 GO:0051726; P:regulation of cell cycle; IMP:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 

Interpro

 IPR023398; TIF_eIF4e-like_dom.
 IPR001040; TIF_eIF_4E.
 IPR019770; TIF_eIF_4E_CS. 

Pfam

 PF01652; IF4E 

SMART

  

PROSITE

 PS00813; IF4E 

PRINTS