CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015269
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/arginine-rich splicing factor 2 
Protein Synonyms/Alias
 Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2 
Gene Name
 Srsf2 
Gene Synonyms/Alias
 Sfrs2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
36TLRRVFEKYGRVGDVacetylation[1]
52IPRDRYTKESRGFAFacetylation[1]
65AFVRFHDKRDAEDAMacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
 Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment (By similarity). 
Sequence Annotation
 DOMAIN 14 92 RRM.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 23 23 Phosphotyrosine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 36 36 N6-acetyllysine (By similarity).
 MOD_RES 52 52 N6-acetyllysine (By similarity).
 MOD_RES 187 187 Phosphoserine (By similarity).
 MOD_RES 189 189 Phosphoserine (By similarity).
 MOD_RES 191 191 Phosphoserine (By similarity).
 MOD_RES 204 204 Phosphoserine (By similarity).
 MOD_RES 206 206 Phosphoserine (By similarity).
 MOD_RES 208 208 Phosphoserine (By similarity).
 MOD_RES 212 212 Phosphoserine (By similarity).
 MOD_RES 220 220 Phosphoserine (By similarity).
 MOD_RES 221 221 Phosphoserine (By similarity).  
Keyword
 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 221 AA 
Protein Sequence
MSYGRPPPDV EGMTSLKVDN LTYRTSPDTL RRVFEKYGRV GDVYIPRDRY TKESRGFAFV 60
RFHDKRDAED AMDAMDGAVL DGRELRVQMA RYGRPPDSHH SRRGPPPRRY GGGGYGRRSR 120
SPRRRRRSRS RSRSRSRSRS RSRYSRSKSR SRTRSRSRST SKSRSARRSK SKSSSVSRSR 180
SRSRSRSRSR SPPPVSKRES KSRSRSKSPP KSPEEEGAVS S 221 
Gene Ontology
 GO:0016607; C:nuclear speck; IEA:Compara.
 GO:0005681; C:spliceosomal complex; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS