CPLM-018922

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-018922

UniProt Accession

AT2A3_HUMAN; Q93084; A8MZG0; D3DTJ8; O60900; O60901; O75501; O75502; Q16115; Q6JHX1; Q8TEX5; Q8TEX6

Genbank Protein ID

Z69881; Z69880; Y15724; Y15725; Y15726; Y15727; Y15728; Y15729; Y15730; Y15738; Y15737; AF068220; AF068221; AC005940; CH471108; CH471108; CH471108; BC035729; S68239; AF458228; AF458229; AY460339

Genbank Nucleotide ID

CAA93737.1; CAA93736.1; CAA75739.1; CAA75739.1; CAA75739.1; CAA75739.1; CAA75739.1; CAA75739.1; CAA75739.1; CAA75748.1; CAA75747.1; AAC24525.1; AAC24526.1; EAW90468.1; EAW90463.1; EAW90469.1; AAH35729.1; AAB29700.1; AAL78967.1; AAL78968.1; AAR15415.1

Protein Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Protein Synonyms/Alias

SERCA3; SR Ca(2+)-ATPase 3; Calcium pump 3

Gene Name

ATP2A3

Gene Synonyms/Alias

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
352	TSVICSDKTGTLTTN	ubiquitination	[1]
476	GACNTVIKQLMRKEF	ubiquitination	[1]
481	VIKQLMRKEFTLEFS	ubiquitination	[1]
492	LEFSRDRKSMSVYCT	ubiquitination	[1]
515	QGSKMFVKGAPESVI	ubiquitination	[1]
548	SREQILAKIRDWGSG	ubiquitination	[1]
629	VMITGDNKGTAVAIC	ubiquitination	[1]
651	DTEDVAGKAYTGREF	ubiquitination	[1]
712	VNDAPALKKAEIGIA	ubiquitination	[1]

Reference

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Sequence Annotation

ACT_SITE 351 351 4-aspartylphosphate intermediate (By
METAL 304 304 Calcium 2; via carbonyl oxygen (By
METAL 305 305 Calcium 2; via carbonyl oxygen (By
METAL 307 307 Calcium 2; via carbonyl oxygen (By
METAL 309 309 Calcium 2 (By similarity).
METAL 703 703 Magnesium (By similarity).
METAL 707 707 Magnesium (By similarity).
METAL 768 768 Calcium 1 (By similarity).
METAL 771 771 Calcium 1 (By similarity).
METAL 796 796 Calcium 2 (By similarity).
METAL 799 799 Calcium 1 (By similarity).
METAL 800 800 Calcium 1 (By similarity).
METAL 800 800 Calcium 2 (By similarity).
METAL 908 908 Calcium 1 (By similarity).
BINDING 515 515 ATP (By similarity).
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 662 662 Phosphoserine.

Keyword

Acetylation; Alternative splicing; ATP-binding; Calcium; Calcium transport; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Sarcoplasmic reticulum; Transmembrane; Transmembrane helix; Transport.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1043 AA

Protein Sequence

MEAAHLLPAA DVLRHFSVTA EGGLSPAQVT GARERYGPNE LPSEEGKSLW ELVLEQFEDL 60
LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 120
EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 180
TGESVSVTKH TEAIPDPRAV NQDKKNMLFS GTNITSGKAV GVAVATGLHT ELGKIRSQMA 240
AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 300
ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 360
MSVCRMFVVA EADAGSCLLH EFTISGTTYT PEGEVRQGDQ PVRCGQFDGL VELATICALC 420
NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLQ ALSRVERAGA CNTVIKQLMR 480
KEFTLEFSRD RKSMSVYCTP TRPHPTGQGS KMFVKGAPES VIERCSSVRV GSRTAPLTPT 540
SREQILAKIR DWGSGSDTLR CLALATRDAP PRKEDMELDD CSKFVQYETD LTFVGCVGML 600
DPPRPEVAAC ITRCYQAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVAG KAYTGREFDD 660
LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 720
GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYSNMKQF IRYLISSNVG EVVCIFLTAI 780
LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKL PRSPREALIS GWLFFRYLAI 840
GVYVGLATVA AATWWFVYDA EGPHINFYQL RNFLKCSEDN PLFAGIDCEV FESRFPTTMA 900
LSVLVTIEMC NALNSVSENQ SLLRMPPWMN PWLLVAVAMS MALHFLILLV PPLPLIFQVT 960
PLSGRQWVVV LQISLPVILL DEALKYLSRN HMHACLYPGL LRTVSQAWSR QPLTTSWTPD 1020
HTGARDTASS RCQSCSEREE AGKK 1044

Gene Ontology

GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
GO:0031095; C:platelet dense tubular network membrane; TAS:Reactome.
GO:0016529; C:sarcoplasmic reticulum; IDA:UniProtKB.
GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0005388; F:calcium-transporting ATPase activity; TAS:ProtInc.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0007596; P:blood coagulation; TAS:Reactome.

Interpro

IPR005782; ATPase_P-typ_Ca-transp_IIA.
IPR006068; ATPase_P-typ_cation-transptr_C.
IPR004014; ATPase_P-typ_cation-transptr_N.
IPR023299; ATPase_P-typ_cyto_domN.
IPR018303; ATPase_P-typ_P_site.
IPR023298; ATPase_P-typ_TM_dom.
IPR008250; ATPase_P-typ_transduc_dom_A.
IPR001757; Cation_transp_P_typ_ATPase.
IPR023214; HAD-like_dom.

Pfam

PF00689; Cation_ATPase_C
PF00690; Cation_ATPase_N
PF00122; E1-E2_ATPase
PF00702; Hydrolase

SMART

SM00831; Cation_ATPase_N

PROSITE

PS00154; ATPASE_E1_E2

PRINTS

PR00119; CATATPASE.
PR00120; HATPASE.