UniProt Accession

 MDH_ECOLI; P61889; O30401; O30402; O30403; P06994; Q2M8X7; Q59343; Q59344; Q59345; Q59346; Q59347; Q59348; Q60133; Q60150; Q933J3; Q93R02; Q9FDQ3; Q9FDQ4; Q9FDQ5 

Genbank Protein ID

 Y00129; M24777; U18997; U00096; AP009048; M10417; U04742; U04743; U04744; U04745; U04746; U04747; U04748; U04749; U04750; U04751; U04752; U04753; U04754; U04755; U04756; U04757; U04758; U04759; U04760; U04770; AF004170; AF004171; AF004172; AF004173; AF004174; AF004175; AF004176; AF004177; AF004179; AF004180; AF004182; AF004183; AF004184; AF004186; AF004187; AF004188; AF004190; AF004191; AF004195; AF004196; AF004199; AF004200; AF004201; AF004202; AF004203; AF004204; AF004205; AF004206; AF004207; AF004208; AF004209; AF091758; AF091759; AF091760; AF091761; AF091762; AF091763; AF091764; AF091765; AF091766; AF091767; AF091768; AF091769; AF091770; AF091771; AF091772; AF091773; AF091774; AF091775; AF091776; AF091777; AF091778 

Genbank Nucleotide ID

 CAA68326.1; AAA16107.1; AAA58038.1; AAC76268.1; BAE77279.1; AAA24147.1; AAC43730.1; AAC43731.1; AAC43732.1; AAC43733.1; AAC43734.1; AAC43735.1; AAC43736.1; AAC43737.1; AAC43738.1; AAC43739.1; AAC43740.1; AAC43741.1; AAC43742.1; AAC43743.1; AAC43744.1; AAC43745.1; AAC43746.1; AAC43747.1; AAC43748.1; AAC43758.1; AAB87003.1; AAB87004.1; AAB87005.1; AAB87006.1; AAB87007.1; AAB87008.1; AAB87009.1; AAB87010.1; AAB87012.1; AAB87013.1; AAB87015.1; AAB87016.1; AAB87017.1; AAB87019.1; AAB87020.1; AAB87021.1; AAB87023.1; AAB87024.1; AAB87028.1; AAB87029.1; AAB87032.1; AAB87033.1; AAB87034.1; AAB87035.1; AAB87036.1; AAB87037.1; AAB87038.1; AAB87039.1; AAB87040.1; AAB87041.1; AAB87042.1; AAF97988.1; AAF97989.1; AAF97990.1; AAF97991.1; AAF97992.1; AAF97993.1; AAF97994.1; AAF97995.1; AAF97996.1; AAF97997.1; AAF97998.1; AAF97999.1; AAF98000.1; AAF98001.1; AAF98002.1; AAF98003.1; AAF98004.1; AAF98005.1; AAF98006.1; AAF98007.1; AAF98008.1 

Protein Name

 Malate dehydrogenase 

Protein Synonyms/Alias

  

Gene Name

 mdh 

Gene Synonyms/Alias

 b3236; JW3205 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
54	SHIPTAVKIKGFSGE	acetylation	[1, 2]
56	IPTAVKIKGFSGEDA	acetylation	[1, 2]
82	ISAGVARKPGMDRSD	acetylation	[1, 2]
99	NVNAGIVKNLVQQVA	acetylation	[1, 2, 3]
107	NLVQQVAKTCPKACI	acetylation	[1, 2]
133	AIAAEVLKKAGVYDK	acetylation	[1, 2]
134	IAAEVLKKAGVYDKN	acetylation	[1, 2]
140	KKAGVYDKNKLFGVT	acetylation	[1, 2, 3]
142	AGVYDKNKLFGVTTL	acetylation	[2]
162	NTFVAELKGKQPGEV	acetylation	[2]
272	SQPLLLGKNGVEERK	acetylation	[1, 2]
279	KNGVEERKSIGTLSA	acetylation	[1, 2]
300	EGMLDTLKKDIALGE	acetylation	[1, 2]
301	GMLDTLKKDIALGEE	acetylation	[1, 2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 Catalyzes the reversible oxidation of malate to oxaloacetate. 

Sequence Annotation

 NP_BIND 7 13 NAD.
 NP_BIND 117 119 NAD.
 ACT_SITE 177 177 Proton acceptor.
 BINDING 34 34 NAD.
 BINDING 81 81 Substrate.
 BINDING 87 87 Substrate.
 BINDING 94 94 NAD.
 BINDING 119 119 Substrate.
 BINDING 153 153 Substrate.
 BINDING 227 227 NAD.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0019898; C:extrinsic to membrane; IDA:EcoliWiki.
 GO:0030060; F:L-malate dehydrogenase activity; IEA:HAMAP.
 GO:0016615; F:malate dehydrogenase activity; IDA:EcoliWiki.
 GO:0009061; P:anaerobic respiration; IDA:EcoliWiki.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006113; P:fermentation; IDA:EcoliWiki.
 GO:0006096; P:glycolysis; IDA:EcoliWiki.
 GO:0006108; P:malate metabolic process; IDA:EcoliWiki.
 GO:0006099; P:tricarboxylic acid cycle; IDA:EcoliWiki. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR010097; Malate_DH_type1.
 IPR023958; Malate_DH_type1_bac.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS