CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000837
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Xaa-Pro aminopeptidase 1 
Protein Synonyms/Alias
 Aminoacylproline aminopeptidase; Cytosolic aminopeptidase P; Soluble aminopeptidase P; sAmp; X-Pro aminopeptidase 1; X-prolyl aminopeptidase 1, soluble; Xaa-Pro aminopeptidase 1, N-terminally processed 
Gene Name
 Xpnpep1 
Gene Synonyms/Alias
 App 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
130IIPTDYWKKMAKVLRacetylation[1]
134DYWKKMAKVLRSAGHacetylation[1]
147GHHLVPVKENLVDKIacetylation[1]
287ADLSPREKVWVSDKAacetylation[1]
293EKVWVSDKASYAVSEacetylation[1]
304AVSEAIPKDHRCCMPacetylation[1]
546VVLVVPAKTKYNFNNubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity). 
Sequence Annotation
 METAL 415 415 Manganese 1 (By similarity).
 METAL 426 426 Manganese 1 (By similarity).
 METAL 426 426 Manganese 2 (By similarity).
 METAL 489 489 Manganese 2 (By similarity).
 METAL 523 523 Manganese 1 (By similarity).
 METAL 523 523 Manganese 2 (By similarity).
 METAL 537 537 Manganese 1 (By similarity).
 METAL 537 537 Manganese 2 (By similarity).
 MOD_RES 304 304 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Aminopeptidase; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Manganese; Metal-binding; Metalloprotease; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 623 AA 
Protein Sequence
MAPKVTSELL RQLRQAMRNS ECVAEPIQAY IIPSGDAHQS EYIAPCDCRR AFVSGFDGSA 60
GTAIITEEHA AMWTDGRYFL QAAKQMDNNW TLMKMGLKDT PTQEDWLVSV LPEGSRVGVD 120
PLIIPTDYWK KMAKVLRSAG HHLVPVKENL VDKIWTDRPE RPCKPLLTLG LDYTGISWKE 180
KVADLRLKMA ERSIVWFVVT ALDEIAWLFN LRGSDVEHNP VFFSYAIIGL ERIMLFIDGD 240
RIDAPGVKQH LLLDLGLEAE YKIQVLPYKS ILSELKTLCA DLSPREKVWV SDKASYAVSE 300
AIPKDHRCCM PYTPICIAKA VKNSAESAGM RRAHIKDAVA LCELFNWLEQ EVPKGGVTEI 360
SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPIP ETNRTLSLDE VYLIDSGAQY 420
KDGTTDVTRT MHFGTPTAYE KECFTYVLKG HIAVSAAVFP TGTKGHLLDS FARSALWDSG 480
LDYLHGTGHG VGSFLNVHEG PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV 540
LVVPAKTKYN FNNRGSLTFE PLTLVPIQTK MIDVDALTDK ECDWLNSYHQ TCRDVIGKEL 600
QTQGRQEALE WLLRETEPIS RQH 623 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0070006; F:metalloaminopeptidase activity; ISS:UniProtKB.
 GO:0010815; P:bradykinin catabolic process; ISS:UniProtKB.
 GO:0006508; P:proteolysis; IDA:UniProtKB. 
Interpro
 IPR000587; Creatinase.
 IPR000994; Pept_M24_structural-domain.
 IPR001131; Peptidase_M24B_aminopep-P_CS. 
Pfam
 PF01321; Creatinase_N
 PF00557; Peptidase_M24 
SMART
  
PROSITE
 PS00491; PROLINE_PEPTIDASE 
PRINTS