UniProt Accession

 RFC5_YEAST; P38251; D6VQ88 

Genbank Protein ID

 U26031; X78993; Z35956; AY693173; BK006936 

Genbank Nucleotide ID

 AAC49065.1; CAA55595.1; CAA85036.1; AAT93192.1; DAA07208.1 

Protein Name

 Replication factor C subunit 5 

Protein Synonyms/Alias

 Replication factor C5; Activator 1 40 kDa subunit 

Gene Name

 RFC5 

Gene Synonyms/Alias

 YBR087W; YBR0810 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
256	KSSSPIIKPDWIIVI	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of ATP-dependent clamp loader (RFC and RFC- like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. 

Sequence Annotation

 NP_BIND 43 51 ATP.
 BINDING 5 5 ATP; via carbonyl oxygen.
 BINDING 17 17 ATP.
 BINDING 231 231 ATP.  

Keyword

 3D-structure; ATP-binding; Cell cycle; Complete proteome; DNA replication; DNA-binding; Nucleotide-binding; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 354 AA 

Protein Sequence

Gene Ontology

 GO:0031390; C:Ctf18 RFC-like complex; IPI:SGD.
 GO:0005663; C:DNA replication factor C complex; IDA:SGD.
 GO:0031391; C:Elg1 RFC-like complex; IPI:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0031389; C:Rad17 RFC-like complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0006272; P:leading strand elongation; IDA:SGD.
 GO:0007062; P:sister chromatid cohesion; IMP:SGD. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR008921; DNA_pol3_clamp-load_cplx_C.
 IPR027417; P-loop_NTPase.
 IPR013748; Rep_factorC_C_dom. 

Pfam

 PF08542; Rep_fac_C 

SMART

 SM00382; AAA 

PROSITE

  

PRINTS