CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010111
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Glycine--tRNA ligase 
Protein Synonyms/Alias
 Glycyl-tRNA synthetase; GlyRS 
Gene Name
 glyQS 
Gene Synonyms/Alias
 glyS; MPN_354; MP482 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
292FDLEQHQKYAKKPLDacetylation[1]
393ESAGSIGKRYRKADAacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Catalyzes the attachment of glycine to tRNA(Gly) (By similarity). 
Sequence Annotation
 NP_BIND 190 192 ATP (By similarity).
 NP_BIND 200 205 ATP (By similarity).
 NP_BIND 275 276 ATP (By similarity).
 NP_BIND 319 322 ATP (By similarity).
 REGION 205 209 Substrate binding (By similarity).
 REGION 315 319 Substrate binding (By similarity).
 BINDING 100 100 Substrate (By similarity).
 BINDING 158 158 Substrate (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 449 AA 
Protein Sequence
MAQVYSQEVY VQYLKRYGFV FQSSELYNGL ANSWDFGPLG AVLKQQIKTA LYNFFIKNKR 60
DVLLIDTPII LNEQIWKASG HLANFTDALV DCKSCKLRFR VDHLDEQIKS ATQWNPKQVN 120
CPNCKANNWS EVRDFNLLFQ TEIGVVNSEK RLVYLRPETA QGIFINFKQL LQLKKRPLPF 180
GVAQFGKSFR NEVTPGNFLF RVREFEQFEM EWFCNPQASL SVFESQQQAI AHFLFKVLQL 240
NPALVKQYEY DKNELAHYAN KTVDFLFQFP HGLRELWGLA DRGTFDLEQH QKYAKKPLDF 300
FDGENNEHFI PAVVEPSVGI ERLFYALIVS SYQQEQLEGE MREVLRLPFH LCPEQIVVLP 360
LVNKLKETAQ TLFEALSQTH WRIGFESAGS IGKRYRKADA IGTKFAITFD FESLEDQAVT 420
IRERDSLKQV RVPIKELKAW FAQHDDQSH 449 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004820; F:glycine-tRNA ligase activity; ISS:UniProtKB.
 GO:0046983; F:protein dimerization activity; ISS:UniProtKB.
 GO:0006426; P:glycyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR027031; Gly-tRNA_synthase/POLG2.
 IPR022961; Gly_tRNA_ligase_bac.
 IPR002315; tRNA-synt_gly. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01043; TRNASYNTHGLY.