CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029439
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Glycogen synthase kinase-3 alpha 
Protein Synonyms/Alias
  
Gene Name
 GSK3A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84RELQIMRKLDHCNIVubiquitination[1]
164GVCHRDIKPQNLLVDubiquitination[1]
178DPDTAVLKLCDFGSAubiquitination[1]
186LCDFGSAKQLVRGEPubiquitination[1, 2, 3]
273NPNYTEFKFPQIKAHubiquitination[1, 4]
278EFKFPQIKAHPWTKVubiquitination[2, 4]
284IKAHPWTKVFKSRTPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 401 AA 
Protein Sequence
MGQWIVYPLC SPGDSGKVTT VVATLGQGPE RSQEVAYTDI KVIGNGSFGV VYQARLAETR 60
ELVAIKKVLQ DKRFKNRELQ IMRKLDHCNI VRLRYFFYSS GEKKDELYLN LVLEYVPETV 120
YRVARHFTKA KLTIPILYVK VYMYQLFRSL AYIHSQGVCH RDIKPQNLLV DPDTAVLKLC 180
DFGSAKQLVR GEPNVSYICS RYYRAPELIF GATDYTSSID VWSAGCVLAE LLLGQPIFPG 240
DSGVDQLVEI IKVLGTPTRE QIREMNPNYT EFKFPQIKAH PWTKVFKSRT PPEAIALCSS 300
LLEYTPSSRL SPLEACAHSF FDELRCLGTQ LPNNRPLPPL FNFSAGELSI QPSLNAILIP 360
PHLRSPAGTT TLTPSSQALT ETPTSSDWQS TDATPTLTNS S 401 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS