UniProt Accession

 BPHL_HUMAN; Q86WA6; Q00306; Q13855; Q3KP51 

Genbank Protein ID

 X81372; AJ617684; AL031963; AL031963; CH471087; BC106901; X57653 

Genbank Nucleotide ID

 CAA57137.1; CAE85120.1; CAD70626.1; CAD70627.1; EAW55122.1; AAI06902.1; CAA40859.1 

Protein Name

 Valacyclovir hydrolase 

Protein Synonyms/Alias

 VACVase; Valacyclovirase; Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Bph-rp; Breast epithelial mucin-associated antigen; MCNAA 

Gene Name

 BPHL 

Gene Synonyms/Alias

 MCNAA 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
87	QLKNLNKKLFTVVAW	ubiquitination	[1, 2]
126	KDAVDLMKALKFKKV	acetylation	[3, 4]
191	KWSERTRKPLEALYG	ubiquitination	[1, 2]
257	FHADFIHKHVKGSRL	acetylation	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. 

Sequence Annotation

 ACT_SITE 139 139 Nucleophile.
 ACT_SITE 244 244 Charge relay system.
 ACT_SITE 272 272 Charge relay system.
 MOD_RES 126 126 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Hydrolase; Reference proteome; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 291 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0006520; P:cellular amino acid metabolic process; TAS:ProtInc.
 GO:0009636; P:response to toxic substance; TAS:ProtInc. 

Interpro

  

Pfam

  

SMART

  

PROSITE

 PS00120; LIPASE_SER 

PRINTS