CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008479
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thiosulfate sulfurtransferase 
Protein Synonyms/Alias
 Rhodanese 
Gene Name
 Tst 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
14YRALVSTKWLAESIRacetylation[1, 2, 3, 4, 5, 6, 7, 8, 9]
14YRALVSTKWLAESIRsuccinylation[8]
14YRALVSTKWLAESIRubiquitination[10]
136GGFRNWLKEGHPVTSacetylation[5, 7, 8, 9, 11]
136GGFRNWLKEGHPVTSsuccinylation[8]
154RPEPAVFKATLNLSLacetylation[7, 8, 9]
154RPEPAVFKATLNLSLsuccinylation[8]
163TLNLSLLKTYEQVLEacetylation[7, 9]
175VLENLQSKRFQLVDSacetylation[5, 7, 8, 9]
175VLENLQSKRFQLVDSsuccinylation[8]
219PFMDFLTKDGFEKSPacetylation[2, 3, 5, 7, 8, 9]
219PFMDFLTKDGFEKSPsuccinylation[8]
219PFMDFLTKDGFEKSPubiquitination[10]
224LTKDGFEKSPEELRAacetylation[5, 7, 8]
224LTKDGFEKSPEELRAsuccinylation[8]
236LRAIFQDKKVDLSQPacetylation[5, 7, 9]
237RAIFQDKKVDLSQPLacetylation[7, 8]
237RAIFQDKKVDLSQPLsuccinylation[8]
293ETRVSQGKSGKA***acetylation[8]
293ETRVSQGKSGKA***succinylation[8]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [9] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [10] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [11] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647
Functional Description
 Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA (By similarity). Formation of iron-sulfur complexes and cyanide detoxification. 
Sequence Annotation
 DOMAIN 25 143 Rhodanese 1.
 DOMAIN 173 288 Rhodanese 2.
 REGION 144 159 Hinge.
 ACT_SITE 248 248 Cysteine persulfide intermediate (By
 BINDING 187 187 Substrate (By similarity).
 BINDING 250 250 Substrate (By similarity).
 MOD_RES 14 14 N6-acetyllysine; alternate (By
 MOD_RES 14 14 N6-succinyllysine; alternate (By
 MOD_RES 136 136 N6-acetyllysine.
 MOD_RES 164 164 Phosphothreonine.
 MOD_RES 165 165 Phosphotyrosine.  
Keyword
 Acetylation; Complete proteome; Mitochondrion; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 297 AA 
Protein Sequence
MVHQVLYRAL VSTKWLAESI RSGRLGPSLR VLDASWYSPG TRQARKEYQE RHVPGASFFD 60
IEECRDTTSP YEMMLPSEAH FGDYVGNLGI SNDTHVVVYD GDDLGSFYAP RVWWMFRVFG 120
HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLNLS LLKTYEQVLE NLQSKRFQLV 180
DSRAQGRYLG TQPEPDIVGL DSGHIRGSVN MPFMDFLTKD GFEKSPEELR AIFQDKKVDL 240
SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPETRVS QGKSGKA 297 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0008097; F:5S rRNA binding; ISS:UniProtKB.
 GO:0004792; F:thiosulfate sulfurtransferase activity; IEA:EC.
 GO:0035928; P:rRNA import into mitochondrion; ISS:UniProtKB. 
Interpro
 IPR001763; Rhodanese-like_dom.
 IPR001307; Thiosulphate_STrfase_CS. 
Pfam
 PF00581; Rhodanese 
SMART
 SM00450; RHOD 
PROSITE
 PS00380; RHODANESE_1
 PS00683; RHODANESE_2
 PS50206; RHODANESE_3 
PRINTS