UniProt Accession

 T2FB_YEAST; P41896; D6VUE2 

Genbank Protein ID

 U13016; Z72790; AY692871; BK006941 

Genbank Nucleotide ID

 AAA61642.1; CAA96988.1; AAT92890.1; DAA08103.1 

Protein Name

 Transcription initiation factor IIF subunit beta 

Protein Synonyms/Alias

 ATP-dependent helicase TFG2; TFIIF medium subunit; TFIIF-beta; Transcription factor G 54 kDa subunit 

Gene Name

 TFG2 

Gene Synonyms/Alias

 YGR005C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
80	LPMFLAEKWRDRNNL	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP- dependent DNA-helicase activity (By similarity). 

Sequence Annotation

 MOD_RES 28 28 Phosphoserine.
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 56 56 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Direct protein sequencing; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 400 AA 

Protein Sequence

Gene Ontology

 GO:0005674; C:transcription factor TFIIF complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000991; F:core RNA polymerase II binding transcription factor activity; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0001111; P:promoter clearance from RNA polymerase II promoter; IC:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IC:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0001174; P:transcriptional start site selection at RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR003196; TFIIF_beta.
 IPR011039; TFIIF_interaction.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02270; TFIIF_beta 

SMART

  

PROSITE

  

PRINTS