UniProt Accession

 NFM_MOUSE; P08553; A2VCT5; Q0VDM8; Q3HRJ6; Q3TNS4; Q3TPK2; Q61961; Q8BQ20 

Genbank Protein ID

 X05640; DQ201636; AK051696; AK164318; AK165041; BC119602; BC128564; M20481 

Genbank Nucleotide ID

 CAA29127.1; ABA46749.1; BAC34724.1; BAE37734.1; BAE38014.1; AAI19603.1; AAI28565.1; AAA39815.1 

Protein Name

 Neurofilament medium polypeptide 

Protein Synonyms/Alias

 NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein 

Gene Name

 Nefm 

Gene Synonyms/Alias

 Nef3; Nfm 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
53	STVSSSYKRSALAPR	ubiquitination	[1]
125	HYLEQQNKEIEAEIQ	ubiquitination	[1]
137	EIQALRQKQASHAQL	ubiquitination	[1]
166	LEMVNHEKAQVQLDS	ubiquitination	[1]
221	VKVELDKKVQSLQDE	ubiquitination	[1]
261	VERKDYLKTDISTAL	ubiquitination	[1]
269	TDISTALKEIRSQLE	ubiquitination	[1]
291	HQAEEWFKCRYAKLT	ubiquitination	[1]
296	WFKCRYAKLTEAAEQ	ubiquitination	[1]
305	TEAAEQNKEAIRSAK	ubiquitination	[1]
312	KEAIRSAKEEIAEYR	ubiquitination	[1]
325	YRRQLQSKSIELESV	ubiquitination	[1]
401	IEIAAYRKLLEGEET	ubiquitination	[1]
434	PSVTISSKIQKTKVE	ubiquitination	[1]
460	EEIIEETKVEDEKSE	ubiquitination	[1]
484	EELAASAKEEKEEAE	ubiquitination	[1]
592	KVEEVAVKEEIKVEK	ubiquitination	[1]
674	AESPVKEKAVEEVIT	ubiquitination	[1]
684	EEVITISKSVKVSLE	ubiquitination	[1]
687	ITISKSVKVSLEKDT	ubiquitination	[1]
803	EGGDGATKYITKSVT	ubiquitination	[1]
807	GATKYITKSVTVTQK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REGION 2 102 Head.
 REGION 103 410 Rod.
 REGION 103 134 Coil 1A.
 REGION 135 147 Linker 1.
 REGION 148 246 Coil 1B.
 REGION 247 263 Linker 12.
 REGION 264 285 Coil 2A.
 REGION 286 289 Linker 2.
 REGION 290 410 Coil 2B.
 REGION 411 848 Tail.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 318 318 Phosphotyrosine.
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 506 506 Phosphoserine.
 MOD_RES 537 537 Phosphoserine (By similarity).
 MOD_RES 545 545 Phosphoserine (By similarity).
 MOD_RES 551 551 Phosphoserine (By similarity).
 MOD_RES 605 605 Phosphoserine.
 MOD_RES 610 610 Phosphoserine.
 MOD_RES 642 642 Phosphothreonine.
 MOD_RES 645 645 Phosphoserine.
 MOD_RES 669 669 Phosphoserine.
 MOD_RES 689 689 Phosphoserine (By similarity).
 MOD_RES 715 715 Phosphoserine.
 MOD_RES 769 769 Phosphoserine.
 CARBOHYD 47 47 O-linked (GlcNAc) (Probable).
 CARBOHYD 430 430 O-linked (GlcNAc) (Probable).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 848 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005883; C:neurofilament; IDA:MGI.
 GO:0031594; C:neuromuscular junction; IDA:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0008088; P:axon cargo transport; IMP:MGI.
 GO:0045110; P:intermediate filament bundle assembly; IGI:MGI.
 GO:0000226; P:microtubule cytoskeleton organization; IGI:MGI.
 GO:0060052; P:neurofilament cytoskeleton organization; IMP:MGI.
 GO:0031133; P:regulation of axon diameter; IMP:MGI. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS.
 IPR002957; Keratin_I. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS

 PR01248; TYPE1KERATIN.