CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008044
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin ligase-binding protein BUL1 
Protein Synonyms/Alias
 Respiration deficiency suppressor 1 
Gene Name
 BUL1 
Gene Synonyms/Alias
 DAG1; RDS1; SMM2; YMR275C; YM8021.01C; YM8156.17C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
302NKSQANLKFEMFYVTubiquitination[1]
558SQEILQRKLDQLHIKubiquitination[2]
578LVNYNDLKLGHDLDNubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other permeases such as PMA1. The RSP5-BUL1/2 complex is also necessary for the heat- shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription and pressure-induced differential regulation of the 2 tryptophan permeases TAT1 and TAT2. 
Sequence Annotation
 MOTIF 156 160 PY-motif.  
Keyword
 Complete proteome; Cytoplasm; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 976 AA 
Protein Sequence
MAKDLNDSGF PPKRKPLLRP QRSDFTANSS TTMNVNANTR GRGRQKQEGG KGSSRSPSLH 60
SPKSWIRSAS ATGILGLRRP ELAHSHSHAP STGTPAGGNR SPLRRSTANA TPVETGRSLT 120
DGDINNVVDV LPSFEMYNTL HRHIPQGNVD PDRHDFPPSY QEANNSTATG AAGSSADLSH 180
QSLSTDALGA TRSSSTSNLE NLIPLRTEHH SIAAHQSTAV DEDSLDIPPI LDDLNDTDNI 240
FIDKLYTLPK MSTPIEITIK TTKHAPIPHV KPEEESILKE YTSGDLIHGF ITIENKSQAN 300
LKFEMFYVTL ESYISIIDKV KSKRTIKRFL RMVDLSASWS YSKIALGSGV DFIPADVDYD 360
GSVFGLNNSR VLEPGVKYKK FFIFKLPLQL LDVTCKQEHF SHCLLPPSFG IDKYRNNCKY 420
SGIKVNRVLG CGHLGTKGSP ILTNDMSDDN LSINYTIDAR IVGKDQKASK LYIMKEREYN 480
LRVIPFGFDA NVVGERTTMS QLNDITKLVQ ERLDALRKIF QRLEKKEPIT NRDIHGADLS 540
GTIDDSIESD SQEILQRKLD QLHIKNRNNY LVNYNDLKLG HDLDNGRSGN SGHNTDTSRA 600
WGPFVESELK YKLKNKSNSS SFLNFSHFLN SSSSSMSSSS NAGKNNHDLT GNKERTGLIL 660
VKAKIPKQGL PYWAPSLLRK TNVFESKSKH DQENWVRLSE LIPEDVKKPL EKLDLQLTCI 720
ESDNSLPHDP PEIQSITTEL ICITAKSDNS IPIKLNSELL MNKEKLTSIK ALYDDFHSKI 780
CEYETKFNKN FLELNELYNM NRGDRRPKEL KFTDFITSQL FNDIESICNL KVSVHNLSNI 840
FKKQVSTLKQ HSKHALSEDS ISHTGNGSSS SPSSASLTPV TSSSKSSLFL PSGSSSTSLK 900
FTDQIVHKWV RIAPLQYKRD INVNLEFNKD IKETLIPSFE SCLCCRFYCV RVMIKFENHL 960
GVAKIDIPIS VRQVTK 976 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IPI:SGD.
 GO:0000151; C:ubiquitin ligase complex; IDA:SGD.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IGI:SGD.
 GO:0000001; P:mitochondrion inheritance; IGI:SGD.
 GO:0006513; P:protein monoubiquitination; IMP:SGD.
 GO:0000209; P:protein polyubiquitination; IPI:SGD. 
Interpro
 IPR022794; Bul1_C.
 IPR007520; Bul1_C_yeast.
 IPR007519; Bul1_N. 
Pfam
 PF04426; Bul1_C
 PF04425; Bul1_N 
SMART
  
PROSITE
  
PRINTS