UniProt Accession

 SET_MOUSE; Q9EQU5; A2BE95; Q9CY82; Q9D0A9; Q9Z181 

Genbank Protein ID

 AB044937; AK011630; AK019960; BX005298; BC018255; AB015613 

Genbank Nucleotide ID

 BAB20793.1; BAB27745.1; BAB31936.1; CAM18951.1; AAH18255.1; BAA34736.1 

Protein Name

 Protein SET 

Protein Synonyms/Alias

 Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I 

Gene Name

 Set 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
11	PTAKASKKELNSNHD	acetylation	[1, 2]
70	LRQPFFQKRSELIAK	ubiquitination	[3]
119	VTEFEDIKSGYRIDF	ubiquitination	[3]
137	ENPYFENKVLSKEFH	ubiquitination	[3]
141	FENKVLSKEFHLNES	ubiquitination	[3]
154	ESGDPSSKSTEIKWK	ubiquitination	[3]
159	SSKSTEIKWKSGKDL	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher (By similarity). 

Sequence Annotation

 REGION 31 77 Dimerization (By similarity).
 REGION 78 224 Earmuff domain (By similarity).
 MOD_RES 2 2 N,N,N-trimethylalanine; by NTM1.
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 131 131 N6-acetyllysine (By similarity).
 MOD_RES 145 145 Phosphotyrosine.
 MOD_RES 149 149 N6-acetyllysine (By similarity).
 MOD_RES 171 171 N6-acetyllysine (By similarity).
 CROSSLNK 153 153 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; DNA-binding; Endoplasmic reticulum; Isopeptide bond; Methylation; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 289 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR002164; NAP_family. 

Pfam

 PF00956; NAP 

SMART

  

PROSITE

  

PRINTS