CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010167
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Translation initiation factor IF-2 
Protein Synonyms/Alias
  
Gene Name
 infB 
Gene Synonyms/Alias
 MPN_155; MP676 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
13PRHFQKNKFDNRAKTacetylation[1]
379FIVFDDEKDAKLIANacetylation[1]
387DAKLIANKFREQQKQacetylation[1]
574EGRVDSLKSEKNPVNacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity). 
Sequence Annotation
 NP_BIND 128 135 GTP (By similarity).
 NP_BIND 175 179 GTP (By similarity).
 NP_BIND 229 232 GTP (By similarity).
 REGION 122 271 G-domain.  
Keyword
 Complete proteome; Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 617 AA 
Protein Sequence
MSKHKPRHFQ KNKFDNRAKT SAKQQFRQVK TGVKDGVFVY KGPLTVSEFC LKTNIPTANI 60
IKHFFLNGVP LTLNSVLSTE QLADACVNFG FDFKVETEIT HDNIISNIKF DDDPTQLSPR 120
PPIVTIMGHV DHGKTSLLDA IRQTNTAAKE FGGITQKIGA YQVKNQEGKT ITFIDTPGHE 180
AFTGMRARGA QVTDIVVLVV AGDDGLKQQT EEAISHAKSA KTPIIVFINK MDKPTANPDM 240
VIQQLNKFDL VPEEWGGDTI FVKGSALTKE GIQELLDSIL LVAEVEDYKA NFNAHSSGYA 300
IEVQTTKGLG PTATIIVKRG TLKIGDIVVL GPAWGKVRTM QDENGVHLQE AMPSKPVQIS 360
GFDIVPVAGE KFIVFDDEKD AKLIANKFRE QQKQKLNTTQ INEELKQKIK SKEIKVLNLI 420
FKVDSDGSLA AIKQAMQSID VPGMSVNIIH SGVGLISEND IMLAKASGAL LFSLNLGLSQ 480
VVKNIASLQG VKVDVHYHIP KLAEEIENIL KGQLEPVYED VELGRAEVLQ LWYHSKVGHI 540
AGTLVKTGKV KRGALCKLLR RNETIYEGRV DSLKSEKNPV NQMEAGKNCG IVINGCEDIQ 600
VCDIILVYEK QEVKSKS 617 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:HAMAP.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR006847; IF2_N.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR000178; TF_IF2_bacterial-like.
 IPR015760; TIF_IF2.
 IPR023115; TIF_IF2_dom3.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF11987; IF-2
 PF04760; IF2_N 
SMART
  
PROSITE
 PS01176; IF2 
PRINTS