CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000249
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein RFXANK 
Protein Synonyms/Alias
 Ankyrin repeat family A protein 1; Regulatory factor X subunit B; RFX-B; Regulatory factor X-associated ankyrin-containing protein 
Gene Name
 RFXANK 
Gene Synonyms/Alias
 ANKRA1; RFXB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107QGELDQLKEHLRKGDubiquitination[1]
112QLKEHLRKGDNLVNKubiquitination[1]
119KGDNLVNKPDERGFTubiquitination[1]
155ADPHILAKERESALSubiquitination[1, 2, 3, 4]
204AVRGNHVKCVEALLAubiquitination[1, 3]
237AVALGYRKVQQVIENubiquitination[1]
248VIENHILKLFQSNLVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. 
Sequence Annotation
 REPEAT 89 118 ANK 1.
 REPEAT 123 152 ANK 2.
 REPEAT 156 185 ANK 3.
 REPEAT 189 218 ANK 4.
 REPEAT 222 251 ANK 5.  
Keyword
 3D-structure; Activator; Alternative splicing; ANK repeat; Complete proteome; Direct protein sequencing; Disease mutation; DNA-binding; Nucleus; Polymorphism; Reference proteome; Repeat; SCID; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 260 AA 
Protein Sequence
MELTQPAEDL IQTQQTPASE LGDPEDPGEE AADGSDTVVL SLFPCTPEPV NPEPDASVSS 60
PQAGSSLKHS TTLTNRQRGN EVSALPATLD SLSIHQLAAQ GELDQLKEHL RKGDNLVNKP 120
DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD 180
VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ 240
VIENHILKLF QSNLVPADPE 260 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:ProtInc.
 GO:0003712; F:transcription cofactor activity; TAS:ProtInc.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR017362; DNA-bd_RFXANK. 
Pfam
 PF00023; Ank 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS