UniProt Accession

 E9PBL0_HUMAN; E9PBL0 

Genbank Protein ID

 AC083829 

Genbank Nucleotide ID

  

Protein Name

 E3 ISG15--protein ligase HERC5 

Protein Synonyms/Alias

  

Gene Name

 HERC5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	TLNEGTVKRWIADVE	ubiquitination	[1, 2]
44	WIADVETKRWQSTKR	ubiquitination	[1, 2]
85	PVYLDLNKARNIFKE	ubiquitination	[1, 2, 3]
281	FNNLSKIKLLHTDTL	ubiquitination	[1, 2]
290	LHTDTLLKIESKKHK	ubiquitination	[1]
505	NKRDYVSKYINYIFN	ubiquitination	[1]
528	EFRRGFYKMCDEDII	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 662 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 

Interpro

 IPR000569; HECT. 

Pfam

 PF00632; HECT 

SMART

 SM00119; HECTc 

PROSITE

 PS50237; HECT 

PRINTS