CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020317
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 10A 
Protein Synonyms/Alias
 EBP50-PDX interactor of 64 kDa; EPI64 protein; Rab27A-GAP-alpha 
Gene Name
 TBC1D10A 
Gene Synonyms/Alias
 EPI64; TBC1D10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
137KLQQNPGKFDELDMSubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A. 
Sequence Annotation
 DOMAIN 111 299 Rab-GAP TBC.
 REGION 505 508 Binding to the PDZ domain of EBP50.
 MOD_RES 40 40 Phosphoserine.
 MOD_RES 407 407 Phosphoserine.  
Keyword
 Alternative splicing; Cell projection; Complete proteome; Direct protein sequencing; GTPase activation; Guanine-nucleotide releasing factor; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MAKSNGENGP RAPAAGESLS GTRESLAQGP DAATTDELSS LGSDSEANGF AERRIDKFGF 60
IVGSQGAEGA LEEVPLEVLR QRESKWLDML NNWDKWMAKK HKKIRLRCQK GIPPSLRGRA 120
WQYLSGGKVK LQQNPGKFDE LDMSPGDPKW LDVIERDLHR QFPFHEMFVS RGGHGQQDLF 180
RVLKAYTLYR PEEGYCQAQA PIAAVLLMHM PAEQAFWCLV QICEKYLPGY YSEKLEAIQL 240
DGEILFSLLQ KVSPVAHKHL SRQKIDPLLY MTEWFMCAFS RTLPWSSVLR VWDMFFCEGV 300
KIIFRVGLVL LKHALGSPEK VKACQGQYET IERLRSLSPK IMQEAFLVQE VVELPVTERQ 360
IEREHLIQLR RWQETRGELQ CRSPPRLHGA KAILDAEPGP RPALQPSPSI RLPLDAPLPG 420
SKAKPKPPKQ AQKEQRKQMK GRGQLEKPPA PNQAMVVAAA GDACPPQHVP PKDSAPKDSA 480
PQDLAPQVSA HHRSQESLTS QESEDTYL 508 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005902; C:microvillus; TAS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0097202; P:activation of cysteine-type endopeptidase activity; IDA:BHF-UCL.
 GO:0045862; P:positive regulation of proteolysis; IDA:BHF-UCL.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS