CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007374
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable phospholipid-transporting ATPase NEO1 
Protein Synonyms/Alias
  
Gene Name
 NEO1 
Gene Synonyms/Alias
 YIL048W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
108LSQDDNFKFTNIASSubiquitination[1]
137YLKVSNTKNWSKFGSubiquitination[1]
141SNTKNWSKFGSPIELubiquitination[1]
705GRKKLNKKIYEQFQKubiquitination[1]
1144LHPPSYAKVQEFATPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Leads to neomycin-resistance when overexpressed. Required for traffic between late Golgi and early endosomes. 
Sequence Annotation
 REGION 1131 1151 Required for endosomal targeting.
 ACT_SITE 503 503 4-aspartylphosphate intermediate
 MOD_RES 102 102 Phosphoserine.
 MOD_RES 551 551 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Endosome; Golgi apparatus; Hydrolase; Magnesium; Membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1151 AA 
Protein Sequence
MPNPPSFKSH KQNLFNSNNN QHANSVDSFD LHLDDSFDAA LDSLQINNNP EPLSKHNTVG 60
DRESFEMRTV DDLDNFSNHS SDSHRKSSNT DTHPLMYDNR LSQDDNFKFT NIASSPPSSS 120
NNIFSKALSY LKVSNTKNWS KFGSPIELSD QHIEREIHPD TTPVYDRNRY VSNELSNAKY 180
NAVTFVPTLL YEQFKFFYNL YFLVVALSQA VPALRIGYLS SYIVPLAFVL TVTMAKEAID 240
DIQRRRRDRE SNNELYHVIT RNRSIPSKDL KVGDLIKVHK GDRIPADLVL LQSSEPSGES 300
FIKTDQLDGE TDWKLRVACP LTQNLSENDL INRISITASA PEKSIHKFLG KVTYKDSTSN 360
PLSVDNTLWA NTVLASSGFC IACVVYTGRD TRQAMNTTTA KVKTGLLELE INSISKILCA 420
CVFALSILLV AFAGFHNDDW YIDILRYLIL FSTIIPVSLR VNLDLAKSVY AHQIEHDKTI 480
PETIVRTSTI PEDLGRIEYL LSDKTGTLTQ NDMQLKKIHL GTVSYTSETL DIVSDYVQSL 540
VSSKNDSLNN SKVALSTTRK DMSFRVRDMI LTLAICHNVT PTFEDDELTY QAASPDEIAI 600
VKFTESVGLS LFKRDRHSIS LLHEHSGKTL NYEILQVFPF NSDSKRMGII VRDEQLDEYW 660
FMQKGADTVM SKIVESNDWL EEETGNMARE GLRTLVIGRK KLNKKIYEQF QKEYNDASLS 720
MLNRDQQMSQ VITKYLEHDL ELLGLTGVED KLQKDVKSSI ELLRNAGIKI WMLTGDKVET 780
ARCVSISAKL ISRGQYVHTI TKVTRPEGAF NQLEYLKINR NACLLIDGES LGMFLKHYEQ 840
EFFDVVVHLP TVIACRCTPQ QKADVALVIR KMTGKRVCCI GDGGNDVSMI QCADVGVGIV 900
GKEGKQASLA ADFSITQFCH LTELLLWHGR NSYKRSAKLA QFVMHRGLII AICQAVYSIC 960
SLFEPIALYQ GWLMVGYATC YTMAPVFSLT LDHDIEESLT KIYPELYKEL TEGKSLSYKT 1020
FFVWVLLSLF QGSVIQLFSQ AFTSLLDTDF TRMVAISFTA LVVNELIMVA LEIYTWNKTM 1080
LVTEIATLLF YIVSVPFLGD YFDLGYMTTV NYYAGLLVIL LISIFPVWTA KAIYRRLHPP 1140
SYAKVQEFAT P 1151 
Gene Ontology
 GO:0005768; C:endosome; IDA:SGD.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015662; F:ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism; ISS:SGD.
 GO:0019829; F:cation-transporting ATPase activity; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004012; F:phospholipid-translocating ATPase activity; ISS:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD.
 GO:0007033; P:vacuole organization; IMP:SGD. 
Interpro
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR006539; ATPase_P-typ_Plipid-transp.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
  
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.