CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030725
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intraflagellar transport protein 122 homolog 
Protein Synonyms/Alias
 cDNA FLJ56621, highly similar to Intraflagellar transport 122 homolog 
Gene Name
 IFT122 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10AVLTWRDKAEHCINDubiquitination[1, 2]
21CINDIAFKPDGTQLIubiquitination[1, 2]
50GTLLQPLKGHKDTVYubiquitination[1, 2, 3]
53LQPLKGHKDTVYCVAubiquitination[1, 2]
121KNGEEKVKIERPGGSubiquitination[1, 2]
156AVADWGQKVSFYQLSubiquitination[1, 2]
165SFYQLSGKQIGKDRAubiquitination[1, 2]
185CCISYFTKGEYILLGubiquitination[1, 2]
196ILLGGSDKQVSLFTKubiquitination[1, 2]
203KQVSLFTKDGVRLGTubiquitination[1]
276QHLITEQKVRIKCKEubiquitination[1, 2]
357CLSFSGVKEREWQMEubiquitination[1, 2]
371ESLIRYIKVIGGPPGubiquitination[1]
421LDMSASRKKLAVVDEubiquitination[1]
422DMSASRKKLAVVDENubiquitination[1]
525YLDRKLFKEAYQIACubiquitination[1]
557GLDFETAKKERKKRGubiquitination[1, 2]
558LDFETAKKERKKRGEubiquitination[1, 2]
581DVFSYQGKFHEAAKLubiquitination[1, 2]
587GKFHEAAKLYKRSGHubiquitination[1, 2]
621FLGSGDPKETKMLITubiquitination[1]
624SGDPKETKMLITKQAubiquitination[1, 2]
629ETKMLITKQADWARNubiquitination[1, 2, 3, 4]
641ARNIKEPKAAVEMYIubiquitination[1]
693LCATYLKKLDSPGYAubiquitination[1, 4]
706YAAETYLKMGDLKSLubiquitination[1, 3]
711YLKMGDLKSLVQLHVubiquitination[1, 2]
864FLLHSLPKDTPSGISubiquitination[1, 2]
872DTPSGISKVKILFTLubiquitination[1, 2]
874PSGISKVKILFTLAKubiquitination[1]
881KILFTLAKQSKALGAubiquitination[1]
884FTLAKQSKALGAYRLubiquitination[1]
898LARHAYDKLRGLYIPubiquitination[1]
910YIPARFQKSIELGTLubiquitination[1, 2, 4, 5]
922GTLTIRAKPFHDSEEubiquitination[1, 2]
1018ILRLVETKDSIGDEDubiquitination[1, 2, 3, 4, 6]
1030DEDPFTAKLSFEQGGubiquitination[1, 2, 3, 6]
1061SRRDVLIKRWPPPLRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
  
Sequence Annotation
  
Keyword
 Cell projection; Cilium; Complete proteome; Flagellum; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1118 AA 
Protein Sequence
MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA 60
YAKDVLCIEG LWSPEQKSVS KHKSSSKIIC CSWTNDGQYL ALGMFNGIIS IRNKNGEEKV 120
KIERPGGSLS PIWSICWNPS REERNDILAV ADWGQKVSFY QLSGKQIGKD RALNFDPCCI 180
SYFTKGEYIL LGGSDKQVSL FTKDGVRLGT VGEQNSWVWT CQAKPDSNYV VVGCQDGTIS 240
FYQLIFSTVH GLYKDRYAYR DSMTDVIVQH LITEQKVRIK CKELVKKIAI YRNRLAIQLP 300
EKILIYELYS EDLSDMHYRV KEKIIKKFEC NLLVVCANHI ILCQEKRLQC LSFSGVKERE 360
WQMESLIRYI KVIGGPPGRE GLLVGLKNGQ ILKIFVDNLF AIVLLKQATA VRCLDMSASR 420
KKLAVVDEND TCLVYDIDTK ELLFQEPNAN SVAWNTQCED MLCFSGGGYL NIKASTFPVH 480
RQKLQGFVVG YNGSKIFCLH VFSISAVEVP QSAPMYQYLD RKLFKEAYQI ACLGVTDTDW 540
RELAMEALEG LDFETAKKER KKRGETNNDL FLADVFSYQG KFHEAAKLYK RSGHENLALE 600
MYTDLCMFEY AKDFLGSGDP KETKMLITKQ ADWARNIKEP KAAVEMYISA GEHVKAIEIC 660
GDHGWVDMLI DIARKLDKAE REPLLLCATY LKKLDSPGYA AETYLKMGDL KSLVQLHVET 720
QRWDEAFALG EKHPEFKDDI YMPYAQWLAE NDRFEEAQKA FHKAGRQREA VQVLEQLTNN 780
AVAESRFNDA AYYYWMLSMQ CLDIAQADPA QKDTMLGKFY HFQRLAELYH GYHAIHRHTE 840
DPFSVHRPET LFNISRFLLH SLPKDTPSGI SKVKILFTLA KQSKALGAYR LARHAYDKLR 900
GLYIPARFQK SIELGTLTIR AKPFHDSEEL VPLCYRCSTN NPLLNNLGNV CINCRQPFIF 960
SASSYDVLHL VEFYLEEGIT DEEAISLIDL EVLRPKRDDR QLEIANNSSQ ILRLVETKDS 1020
IGDEDPFTAK LSFEQGGSEF VPVVVSRLVL RSMSRRDVLI KRWPPPLRWQ YFRSLLPDAS 1080
ITMCPSCFQM FHSEDYELLV LQHGCCPYCR RCKDDPGP 1118 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA. 
Interpro
 IPR011044; Quino_amine_DH_bsu.
 IPR011990; TPR-like_helical.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
  
PRINTS