UniProt Accession

 RNH_ECOLI; P0A7Y4; P00647; Q8FKY5 

Genbank Protein ID

 K00985; V00337; X04027; U70214; U00096; AP009048 

Genbank Nucleotide ID

 AAA24565.1; CAA23620.1; CAA27660.1; AAB08636.1; AAC73319.1; BAA77885.1 

Protein Name

 Ribonuclease HI 

Protein Synonyms/Alias

 RNase HI; Ribonuclease H; RNase H 

Gene Name

 rnhA 

Gene Synonyms/Alias

 dasF; herA; rnh; sdrA; b0214; JW0204 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
33	LRYRGREKTFSAGYT	acetylation	[1]
86	TQWIHNWKKRGWKTA	acetylation	[1]
96	GWKTADKKPVKNVDL	acetylation	[1]
99	TADKKPVKNVDLWQR	acetylation	[1]
117	ALGQHQIKWEWVKGH	acetylation	[1]
122	QIKWEWVKGHAGHPE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Endonuclease that specifically degrades the RNA of RNA- DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. 

Sequence Annotation

 DOMAIN 1 142 RNase H.
 METAL 10 10 Magnesium 1.
 METAL 10 10 Magnesium 2.
 METAL 48 48 Magnesium 1.
 METAL 70 70 Magnesium 1.
 METAL 134 134 Magnesium 2.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 155 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0003676; F:nucleic acid binding; IDA:EcoliWiki.
 GO:0004523; F:ribonuclease H activity; IDA:EcoCyc.
 GO:0043137; P:DNA replication, removal of RNA primer; IMP:EcoCyc. 

Interpro

 IPR022892; RNaseH.
 IPR012337; RNaseH-like_dom.
 IPR002156; RNaseH_domain. 

Pfam

 PF00075; RNase_H 

SMART

  

PROSITE

 PS50879; RNASE_H 

PRINTS