CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009139
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 L-gulonolactone oxidase 
Protein Synonyms/Alias
 LGO; L-gulono-gamma-lactone oxidase; GLO 
Gene Name
 Gulo 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
6**MVHGYKGVQFQNWubiquitination[1]
71GFMIHMGKMNRVLQVubiquitination[1]
80NRVLQVDKEKKQVTVacetylation[2, 3, 4]
82VLQVDKEKKQVTVEAacetylation[2]
83LQVDKEKKQVTVEAGubiquitination[1]
211DNLDSHLKKSEYFRFubiquitination[1]
294ESSNLSHKIFSYECRubiquitination[1]
303FSYECRFKQHVQDWAacetylation[2]
303FSYECRFKQHVQDWAubiquitination[1]
317AIPREKTKEALLELKacetylation[4]
317AIPREKTKEALLELKubiquitination[1]
332AMLEAHPKVVAHYPVubiquitination[1]
398GGRPHWAKAHNCTRKacetylation[2, 5]
398GGRPHWAKAHNCTRKsuccinylation[5]
409CTRKDFEKMYPAFHKacetylation[2]
409CTRKDFEKMYPAFHKubiquitination[1]
416KMYPAFHKFCDIREKacetylation[2]
416KMYPAFHKFCDIREKubiquitination[1]
423KFCDIREKLDPTGMFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
 Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L- ascorbate. 
Sequence Annotation
 DOMAIN 17 187 FAD-binding PCMH-type.
 MOD_RES 54 54 Pros-8alpha-FAD histidine (By  
Keyword
 Ascorbate biosynthesis; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; Membrane; Microsome; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 440 AA 
Protein Sequence
MVHGYKGVQF QNWAKTYGCS PEMYYQPTSV GEVREVLALA RQQNKKVKVV GGGHSPSDIA 60
CTDGFMIHMG KMNRVLQVDK EKKQVTVEAG ILLTDLHPQL DKHGLALSNL GAVSDVTVGG 120
VIGSGTHNTG IKHGILATQV VALTLMKADG TVLECSESSN ADVFQAARVH LGCLGVILTV 180
TLQCVPQFHL LETSFPSTLK EVLDNLDSHL KKSEYFRFLW FPHSENVSII YQDHTNKEPS 240
SASNWFWDYA IGFYLLEFLL WTSTYLPRLV GWINRFFFWL LFNCKKESSN LSHKIFSYEC 300
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL LSPCFQRDSC 360
YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH NCTRKDFEKM YPAFHKFCDI 420
REKLDPTGMF LNSYLEKVFY 440 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0003885; F:D-arabinono-1,4-lactone oxidase activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:UniProtKB.
 GO:0050105; F:L-gulonolactone oxidase activity; IDA:UniProtKB.
 GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:InterPro.
 GO:0019853; P:L-ascorbic acid biosynthetic process; IDA:UniProtKB. 
Interpro
 IPR007173; ALO.
 IPR016169; CO_DH_flavot_FAD-bd_sub2.
 IPR016166; FAD-bd_2.
 IPR016167; FAD-bd_2_sub1.
 IPR010031; FAD_lactone_oxidase.
 IPR010032; FAD_linked_OxRdtase_bac.
 IPR023595; LGO_GLO.
 IPR006094; Oxid_FAD_bind_N.
 IPR006093; Oxy_OxRdtase_FAD_BS. 
Pfam
 PF04030; ALO
 PF01565; FAD_binding_4 
SMART
  
PROSITE
 PS51387; FAD_PCMH
 PS00862; OX2_COVAL_FAD 
PRINTS