UniProt Accession

 CYTB_MOUSE; Q62426; Q3UAW2 

Genbank Protein ID

 U59807; AK002403; AK151205; BC056170 

Genbank Nucleotide ID

 AAC52851.1; BAB22074.1; BAE30202.1; AAH56170.1 

Protein Name

 Cystatin-B 

Protein Synonyms/Alias

 Stefin-B 

Gene Name

 Cstb 

Gene Synonyms/Alias

 Cst6; Stfb 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
34	LESKENQKFDVFKAI	acetylation	[1, 2]
34	LESKENQKFDVFKAI	succinylation	[2]
39	NQKFDVFKAISFKRQ	acetylation	[1]
39	NQKFDVFKAISFKRQ	ubiquitination	[3]
44	VFKAISFKRQIVAGT	acetylation	[1]
63	KVDVGGDKCVHLRVF	acetylation	[1]
78	QPLPHENKPLTLSSY	acetylation	[1, 2, 4, 5]
78	QPLPHENKPLTLSSY	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 This is an intracellular thiol proteinase inhibitor. 

Sequence Annotation

 MOTIF 46 50 Secondary area of contact (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Protease inhibitor; Reference proteome; Thiol protease inhibitor. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 98 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
 GO:0008344; P:adult locomotory behavior; IMP:MGI.
 GO:0010951; P:negative regulation of endopeptidase activity; IEA:GOC.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara. 

Interpro

 IPR000010; Prot_inh_cystat.
 IPR018073; Prot_inh_cystat_CS.
 IPR001713; Prot_inh_stefinA. 

Pfam

 PF00031; Cystatin 

SMART

 SM00043; CY 

PROSITE

 PS00287; CYSTATIN 

PRINTS

 PR00295; STEFINA.