CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000875
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation factor SPT5 
Protein Synonyms/Alias
 DRB sensitivity-inducing factor large subunit; DSIF large subunit 
Gene Name
 Supt5h 
Gene Synonyms/Alias
 Supt5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
211TDTPLQIKSVVAPEHubiquitination[1]
648LVLAGGSKPRDVTNLubiquitination[1]
712RISQGPYKGYIGVVKacetylation[2]
712RISQGPYKGYIGVVKubiquitination[1]
719KGYIGVVKDATESTAubiquitination[1]
1011GMCSVYLKDSEKVVSacetylation[3]
1031LEPITPTKNNKVKVIubiquitination[1]
1078LNLRFLGKLLEA***acetylation[4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity). 
Sequence Annotation
 DOMAIN 271 304 KOW 1.
 DOMAIN 418 449 KOW 2.
 DOMAIN 470 501 KOW 3.
 DOMAIN 592 625 KOW 4.
 DOMAIN 698 731 KOW 5.
 REPEAT 748 753 CTR1-1; approximate.
 REPEAT 754 759 CTR1-2.
 REPEAT 760 765 CTR1-3.
 REPEAT 766 772 CTR1-4.
 REPEAT 775 781 CTR1-5.
 REPEAT 782 788 CTR1-6.
 REPEAT 790 796 CTR1-7.
 REPEAT 797 803 CTR1-8.
 REPEAT 805 811 CTR1-9.
 REPEAT 838 845 CTR2-1.
 REPEAT 848 856 CTR2-2; approximate.
 REPEAT 857 863 CTR2-3; approximate.
 REPEAT 875 879 CTR2-4; half-length.
 REPEAT 890 896 CTR2-5; approximate.
 REPEAT 898 905 CTR2-6.
 REPEAT 910 915 CTR2-7; approximate.
 REPEAT 918 924 CTR2-8.
 REPEAT 926 933 CTR2-9.
 REPEAT 937 944 CTR2-10.
 REGION 174 268 Interaction with SUPT4H1 and SUPT4H2 (By
 REGION 311 418 Interaction with RNA polymerase II (By
 REGION 748 811 9 X 7 AA approximate tandem repeats of G-
 REGION 838 944 10 X 8 AA approximate tandem repeats of
 MOD_RES 664 664 Phosphoserine (By similarity).
 MOD_RES 690 690 Asymmetric dimethylarginine; alternate
 MOD_RES 690 690 Omega-N-methylarginine; alternate (By
 MOD_RES 690 690 Omega-N-methylated arginine; alternate
 MOD_RES 692 692 Asymmetric dimethylarginine; alternate
 MOD_RES 692 692 Omega-N-methylarginine; alternate (By
 MOD_RES 692 692 Omega-N-methylated arginine; alternate
 MOD_RES 692 692 Symmetric dimethylarginine; alternate (By
 MOD_RES 769 769 Phosphothreonine; by CDK9 (By
 MOD_RES 778 778 Phosphothreonine; by CDK9 (By
 MOD_RES 793 793 Phosphothreonine.
 MOD_RES 1028 1028 Phosphothreonine (By similarity).  
Keyword
 Activator; Alternative splicing; Complete proteome; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1082 AA 
Protein Sequence
MSDSEDSNFS EEEDSERSSE AEEAEVEEDQ RSAAGSEKEE EPEEEEEEEE EYDEEEEEED 60
DDRPPKKPRH GGFILDEADV DDEYEDEDQW EDGAEDILEK EEIEASNIDN VVLDEDRSGA 120
RRLQNLWRDQ REEELGEYYM KKYAKSSVGE TVYGGSDELS DDITQQQLLP GVKDPNLWTV 180
KCKIGEERAT AISLMRKFIA YQFTDTPLQI KSVVAPEHVK GYIYVEAYKQ THVKQAIEGV 240
GNLRLGYWNQ QMVPIKEMTD VLKVVKEVAN LKPKSWVRLK RGIYKDDIAQ VDYVEPSQNT 300
ISLKMIPRID YDRIKARMSL KDWFAKRKKF KRPPQRLFDA EKIRSLGGDV ASDGDFLIFE 360
GNRYSRKGFL FKSFAMSAVI TEGVKPTLSE LEKFEDQPEG IDLEVVTEST GKEREHNFQP 420
GDNVEVCEGE LINLQGKVLS VDGNKITIMP KHEDLKDMLE FPAQELRKYF KMGDHVKVIA 480
GRFEGDTGLI VRVEENFVIL FSDLTMHELK VLPRDLQLCS ETASGVDVGG QHEWGELVQL 540
DPRTVGVIVR LERETFQVLN MHGKVVTVRH QAVTQKKDNR FAVALDSDQN NIHVKDIVKV 600
IDGPHSGREG EIRHLYRSFA FLHCKKLVEN GGMFVCKARH LVLAGGSKPR DVTNLTVGGF 660
TPMSPRISSP MHPSAEGQHG GFGSPGGMSR GRGRRDNELI GQTVRISQGP YKGYIGVVKD 720
ATESTARVEL HSTCQTISVD RQRLTTVDSQ RPGGMTSTYG RTPMYGSQTP MYGSGSRTPM 780
YGSQTPLQDG SRTPHYGSQT PLHDGSRTPA QSGAWDPNNP NTPSRAEEEY EYAFDDEPTP 840
SPQAYGGTPN PQTPGYPDPS SPQVNPQYNP QTPGTPAMYN TDQFSPYAAP SPQGSYQPSP 900
SPQSYHQVAP SPAGYQNTHS PASYHPTPSP MAYQASPSPS PVGYSPMTPG APSPGGYNPH 960
TPGSGIEQNS SDWVTTDIQV KVRDTYLDTQ IVGQTGVIRS VTGGMCSVYL KDSEKVVSIS 1020
SEHLEPITPT KNNKVKVILG EDREATGVLL SIDGEDGIIR MDLEDQQIKI LNLRFLGKLL 1080
EA 1082 
Gene Ontology
 GO:0032044; C:DSIF complex; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0032785; P:negative regulation of DNA-dependent transcription, elongation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0016239; P:positive regulation of macroautophagy; IEA:Compara.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IEA:Compara. 
Interpro
 IPR005824; KOW.
 IPR024945; Spt5_C_dom.
 IPR022581; Spt5_N.
 IPR017071; TF_Spt5.
 IPR005100; TF_Spt5_NGN-domain.
 IPR006645; Transcrpt_antiterm_NusG_N.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF00467; KOW
 PF03439; Spt5-NGN
 PF11942; Spt5_N 
SMART
 SM01104; CTD
 SM00739; KOW
 SM00738; NGN 
PROSITE
  
PRINTS