| Tag | Content |
|---|
| CPLM ID | CPLM-001035 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Acetyl-coenzyme A synthetase |
Protein Synonyms/Alias | AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme |
Gene Name | acsA |
Gene Synonyms/Alias | acs; Rv3667; MT3768; MTV025.015 |
Created Date | July 27, 2013 |
Organism | Mycobacterium tuberculosis |
NCBI Taxa ID | 1773 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 617 | LPKTRSGKIMRRLLR | acetylation | [1] |
|
Reference | [1] Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP. Xu H, Hegde SS, Blanchard JS. Biochemistry. 2011 Jul 5;50(26):5883-92. [ PMID: 21627103] |
Functional Description | Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. M.tuberculosis may use AcsA for both acetate and propionate assimilation. |
Sequence Annotation | REGION 190 193 Coenzyme A (By similarity).
REGION 411 416 Substrate binding (By similarity).
ACT_SITE 525 525 By similarity.
METAL 545 545 Magnesium; via carbonyl oxygen (By
METAL 547 547 Magnesium; via carbonyl oxygen (By
BINDING 311 311 Coenzyme A (By similarity).
BINDING 387 387 Substrate; via amide nitrogen (By
BINDING 508 508 Substrate (By similarity).
BINDING 523 523 Substrate (By similarity).
BINDING 531 531 Coenzyme A (By similarity).
BINDING 534 534 Substrate (By similarity).
MOD_RES 617 617 N6-acetyllysine. |
Keyword | Acetylation; ATP-binding; Complete proteome; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 651 AA |
Protein Sequence | MSESTPEVSS SYPPPAHFAE HANARAELYR EAEEDRLAFW AKQANRLSWT TPFTEVLDWS 60
GAPFAKWFVG GELNVAYNCV DRHVEAGHGD RVAIHWEGEP VGDRRTLTYS DLLAEVSKAA 120
NALTDLGLVA GDRVAIYLPL IPEAVIAMLA CARLGIMHSV VFGGFTAAAL QARIVDAQAK 180
LLITADGQFR RGKPSPLKAA ADEALAAIPD CSVEHVLVVR RTGIEMAWSE GRDLWWHHVV 240
GSASPAHTPE PFDSEHPLFL LYTSGTTGKP KGIMHTSGGY LTQCCYTMRT IFDVKPDSDV 300
FWCTADIGWV TGHTYGVYGP LCNGVTEVLY EGTPDTPDRH RHFQIIEKYG VTIYYTAPTL 360
IRMFMKWGRE IPDSHDLSSL RLLGSVGEPI NPEAWRWYRD VIGGGRTPLV DTWWQTETGS 420
AMISPLPGIA AAKPGSAMTP LPGISAKIVD DHGDPLPPHT EGAQHVTGYL VLDQPWPSML 480
RGIWGDPARY WHSYWSKFSD KGYYFAGDGA RIDPDGAIWV LGRIDDVMNV SGHRISTAEV 540
ESALVAHSGV AEAAVVGVTD ETTTQAICAF VVLRANYAPH DRTAEELRTE VARVISPIAR 600
PRDVHVVPEL PKTRSGKIMR RLLRDVAENR ELGDTSTLLD PTVFDAIRAA K 651 |
Gene Ontology | |
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Pfam | |
SMART | |
PROSITE | |
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