UniProt Accession

 SIR2_MACFA; Q4R834 

Genbank Protein ID

 AB168626 

Genbank Nucleotide ID

 BAE00738.1 

Protein Name

 NAD-dependent protein deacetylase sirtuin-2 

Protein Synonyms/Alias

 Regulatory protein SIR2 homolog 2; SIR2-like protein 2 

Gene Name

 SIRT2 

Gene Synonyms/Alias

 QtsA-13614 

Created Date

 July 27, 2013 

Organism

 Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) 

NCBI Taxa ID

 9541 

Lysine Modification

Position	Peptide	Type	References
55	TLSLGSQKERLLDEL	ubiquitination	[1]
126	FEISYFKKHPEPFFA	ubiquitination	[1]
158	FMRLLKDKGLLLRCY	ubiquitination	[1]
212	PLSWMKEKIFSEVTP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA (By similarity). 

Sequence Annotation

 DOMAIN 65 340 Deacetylase sirtuin-type.
 NP_BIND 84 104 NAD (By similarity).
 NP_BIND 167 170 NAD (By similarity).
 NP_BIND 261 263 NAD (By similarity).
 NP_BIND 286 288 NAD (By similarity).
 ACT_SITE 187 187 Proton acceptor (By similarity).
 METAL 195 195 Zinc (By similarity).
 METAL 200 200 Zinc (By similarity).
 METAL 221 221 Zinc (By similarity).
 METAL 224 224 Zinc (By similarity).
 BINDING 324 324 NAD; via amide nitrogen (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 23 23 Phosphoserine (By similarity).  

Keyword

 Acetylation; Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Hydrolase; Metal-binding; Microtubule; Mitosis; NAD; Phosphoprotein; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 389 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
 GO:0070403; F:NAD+ binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0006476; P:protein deacetylation; IEA:InterPro. 

Interpro

 IPR017328; NAD-dep_deAcase_SIR2_class_I.
 IPR003000; Sirtuin.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS