CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001675
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Echinoderm microtubule-associated protein-like 2 
Protein Synonyms/Alias
 EMAP-2; HuEMAP-2 
Gene Name
 EML2 
Gene Synonyms/Alias
 EMAP2; EMAPL2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56ELPSCRLKLEWVYGYubiquitination[1]
327LWGSDYSKLQEVEVPubiquitination[2, 3]
399FVTCGQDKLVHLWSSubiquitination[1]
586VARSHDGKLLASADDubiquitination[1, 2, 3]
596ASADDFGKVHLFSYPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic (By similarity). 
Sequence Annotation
 REPEAT 103 152 WD 1.
 REPEAT 157 200 WD 2.
 REPEAT 203 242 WD 3.
 REPEAT 250 288 WD 4.
 REPEAT 292 331 WD 5.
 REPEAT 375 414 WD 6.
 REPEAT 416 455 WD 7.
 REPEAT 458 497 WD 8.
 REPEAT 504 543 WD 9.
 REPEAT 571 610 WD 10.
 REPEAT 616 648 WD 11.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 649 AA 
Protein Sequence
MSSFGAGKTK EVIFSVEDGS VKMFLRGRPV PMMIPDELAP TYSLDTRSEL PSCRLKLEWV 60
YGYRGRDCRA NLYLLPTGEI VYFVASVAVL YSVEEQRQRH YLGHNDDIKC LAIHPDMVTI 120
ATGQVAGTTK EGKPLPPHVR IWDSVSLSTL HVLGLGVFDR AVCCVGFSKS NGGNLLCAVD 180
ESNDHMLSVW DWAKETKVVD VKCSNEAVLV ATFHPTDPTV LITCGKSHIY FWTLEGGSLS 240
KRQGLFEKHE KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQAVL GAHDGGVFGL 300
CALRDGTLVS GGGRDRRVVL WGSDYSKLQE VEVPEDFGPV RTVAEGHGDT LYVGTTRNSI 360
LQGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL VHLWSSDSHQ PLWSRIIEDP 420
ARSAGFHPSG SVLAVGTVTG RWLLLDTETH DLVAIHTDGN EQISVVSFSP DGAYLAVGSH 480
DNLVYVYTVD QGGRKVSRLG KCSGHSSFIT HLDWAQDSSC FVTNSGDYEI LYWDPATCKQ 540
ITSADAVRNM EWATATCVLG FGVFGIWSEG ADGTDINAVA RSHDGKLLAS ADDFGKVHLF 600
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVV 649 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; TAS:ProtInc.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0008152; P:metabolic process; IEA:GOC.
 GO:0007605; P:sensory perception of sound; TAS:ProtInc.
 GO:0007601; P:visual perception; TAS:ProtInc. 
Interpro
 IPR005108; HELP.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR011041; Quinoprot_gluc/sorb_DH.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF03451; HELP
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS