| Tag | Content |
|---|
| CPLM ID | CPLM-002082 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA-directed RNA polymerase III subunit RPC1 |
Protein Synonyms/Alias | RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; RNA polymerase III subunit C160 |
Gene Name | RPO31 |
Gene Synonyms/Alias | RPC1; RPC160; YOR116C; O3254; YOR3254C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 1240 | NVFPEGYKAKSISTS | ubiquitination | [1] | | 1242 | FPEGYKAKSISTSAK | ubiquitination | [1] | | 1249 | KSISTSAKEPSENDV | ubiquitination | [1] | | 1273 | ALPDVVVKGLPDISR | ubiquitination | [1] | | 1432 | TGSFKVVKGTNISEK | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). |
Sequence Annotation | REGION 858 870 Bridging helix (By similarity).
METAL 67 67 Zinc 1 (By similarity).
METAL 70 70 Zinc 1 (By similarity).
METAL 77 77 Zinc 1 (By similarity).
METAL 80 80 Zinc 1 (By similarity).
METAL 107 107 Zinc 2 (By similarity).
METAL 110 110 Zinc 2 (By similarity).
METAL 154 154 Zinc 2 (By similarity).
METAL 511 511 Magnesium; catalytic (By similarity).
METAL 513 513 Magnesium; catalytic (By similarity).
METAL 515 515 Magnesium; catalytic (By similarity). |
Keyword | Complete proteome; DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Reference proteome; Transcription; Transferase; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1460 AA |
Protein Sequence | MKEVVVSETP KRIKGLEFSA LSAADIVAQS EVEVSTRDLF DLEKDRAPKA NGALDPKMGV 60
SSSSLECATC HGNLASCHGH FGHLKLALPV FHIGYFKATI QILQGICKNC SAILLSETDK 120
RQFLHELRRP GVDNLRRMGI LKKILDQCKK QRRCLHCGAL NGVVKKAAAG AGSAALKIIH 180
DTFRWVGKKS APEKDIWVGE WKEVLAHNPE LERYVKRCMD DLNPLKTLNL FKQIKSADCE 240
LLGIDATVPS GRPETYIWRY LPAPPVCIRP SVMMQDSPAS NEDDLTVKLT EIVWTSSLIK 300
AGLDKGISIN NMMEHWDYLQ LTVAMYINSD SVNPAMLPGS SNGGGKVKPI RGFCQRLKGK 360
QGRFRGNLSG KRVDFSGRTV ISPDPNLSID EVAVPDRVAK VLTYPEKVTR YNRHKLQELI 420
VNGPNVHPGA NYLLKRNEDA RRNLRYGDRM KLAKNLQIGD VVERHLEDGD VVLFNRQPSL 480
HRLSILSHYA KIRPWRTFRL NECVCTPYNA DFDGDEMNLH VPQTEEARAE AINLMGVKNN 540
LLTPKSGEPI IAATQDFITG SYLISHKDSF YDRATLTQLL SMMSDGIEHF DIPPPAIMKP 600
YYLWTGKQVF SLLIKPNHNS PVVINLDAKN KVFVPPKSKS LPNEMSQNDG FVIIRGSQIL 660
SGVMDKSVLG DGKKHSVFYT ILRDYGPQEA ANAMNRMAKL CARFLGNRGF SIGINDVTPA 720
DDLKQKKEEL VEIAYHKCDE LITLFNKGEL ETQPGCNEEQ TLEAKIGGLL SKVREEVGDV 780
CINELDNWNA PLIMATCGSK GSTLNVSQMV AVVGQQIISG NRVPDGFQDR SLPHFPKNSK 840
TPQSKGFVRN SFFSGLSPPE FLFHAISGRE GLVDTAVKTA ETGYMSRRLM KSLEDLSCQY 900
DNTVRTSANG IVQFTYGGDG LDPLEMEGNA QPVNFNRSWD HAYNITFNNQ DKGLLPYAIM 960
ETANEILGPL EERLVRYDNS GCLVKREDLN KAEYVDQYDA ERDFYHSLRE YINGKATALA 1020
NLRKSRGMLG LLEPPAKELQ GIDPDETVPD NVKTSVSQLY RISEKSVRKF LEIALFKYRK 1080
ARLEPGTAIG AIGAQSIGEP GTQMTLKTFH FAGVASMNVT LGVPRIKEII NASKVISTPI 1140
INAVLVNDND ERAARVVKGR VEKTLLSDVA FYVQDVYKDN LSFIQVRIDL GTIDKLQLEL 1200
TIEDIAVAIT RASKLKIQAS DVNIIGKDRI AINVFPEGYK AKSISTSAKE PSENDVFYRM 1260
QQLRRALPDV VVKGLPDISR AVINIRDDGK RELLVEGYGL RDVMCTDGVI GSRTTTNHVL 1320
EVFSVLGIEA ARYSIIREIN YTMSNHGMSV DPRHIQLLGD VMTYKGEVLG ITRFGLSKMR 1380
DSVLQLASFE KTTDHLFDAA FYMKKDAVEG VSECIILGQT MSIGTGSFKV VKGTNISEKD 1440
LVPKRCLFES LSNEAALKAN 1460 |
Gene Ontology | GO:0005666; C:DNA-directed RNA polymerase III complex; IDA:SGD. GO:0003677; F:DNA binding; IEA:InterPro. GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW. GO:0032549; F:ribonucleoside binding; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |