UniProt Accession

 NFM_HUMAN; P07197; B4DGN2; E9PBF7; Q4QRK6 

Genbank Protein ID

 Y00067; EF560736; AK294681; AF106564; CH471080; BC096757 

Genbank Nucleotide ID

 CAA68276.1; ABQ59046.1; BAG57843.1; EAW63602.1; AAH96757.1 

Protein Name

 Neurofilament medium polypeptide 

Protein Synonyms/Alias

 NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein 

Gene Name

 NEFM 

Gene Synonyms/Alias

 NEF3; NFM 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
118	RFAGYIEKVHYLEQQ	ubiquitination	[1, 2]
259	SHITVERKDYLKTDI	ubiquitination	[1, 2]
263	VERKDYLKTDISTAL	ubiquitination	[1, 2]
271	TDISTALKEIRSQLE	ubiquitination	[1, 2]
451	PKLKVQHKFVEEIIE	ubiquitination	[1, 2]
751	AEVVTITKSVKVHLE	ubiquitination	[1, 2]
871	EGGDGATKYITKSVT	ubiquitination	[1, 2]
875	GATKYITKSVTVTQK	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REPEAT 614 626 1.
 REPEAT 627 639 2.
 REPEAT 640 652 3.
 REPEAT 653 665 4.
 REPEAT 666 678 5.
 REPEAT 679 691 6.
 REGION 2 104 Head.
 REGION 105 412 Rod.
 REGION 105 136 Coil 1A.
 REGION 137 149 Linker 1.
 REGION 150 248 Coil 1B.
 REGION 249 265 Linker 12.
 REGION 266 287 Coil 2A.
 REGION 288 291 Linker 2.
 REGION 292 412 Coil 2B.
 REGION 413 916 Tail.
 REGION 614 691 6 X 13 AA approximate tandem repeats of
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 320 320 Phosphotyrosine (By similarity).
 MOD_RES 511 511 Phosphoserine (By similarity).
 MOD_RES 545 545 Phosphoserine (By similarity).
 MOD_RES 553 553 Phosphoserine (By similarity).
 MOD_RES 559 559 Phosphoserine (By similarity).
 MOD_RES 641 641 Phosphoserine (By similarity).
 MOD_RES 646 646 Phosphoserine (By similarity).
 MOD_RES 680 680 Phosphoserine (By similarity).
 MOD_RES 685 685 Phosphoserine (By similarity).
 MOD_RES 736 736 Phosphoserine (By similarity).
 MOD_RES 783 783 Phosphoserine (By similarity).
 MOD_RES 837 837 Phosphoserine.
 CARBOHYD 47 47 O-linked (GlcNAc) (By similarity).
 CARBOHYD 431 431 O-linked (GlcNAc) (By similarity).  

Keyword

 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 916 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005883; C:neurofilament; TAS:ProtInc.
 GO:0031594; C:neuromuscular junction; IEA:Compara.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0008088; P:axon cargo transport; IEA:Compara.
 GO:0045110; P:intermediate filament bundle assembly; IEA:Compara.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0060052; P:neurofilament cytoskeleton organization; IEA:Compara.
 GO:0031133; P:regulation of axon diameter; IEA:Compara. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS.
 IPR002957; Keratin_I. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS

 PR01248; TYPE1KERATIN.