CPLM-022033

Genbank Nucleotide ID

Integral membrane protein 2C

Protein Synonyms/Alias

Cerebral protein 14; Transmembrane protein BRI3; CT-BRI3

BRI3; hucep-14; NPD018; PSEC0047

Homo sapiens (Human)

Position	Peptide	Type	References
3	*****MVKISFQPAV	ubiquitination	[1, 2, 3, 4]
14	QPAVAGIKGDKADKA	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
17	VAGIKGDKADKASAS	ubiquitination	[2, 3, 4, 5, 6, 8]
20	IKGDKADKASASAPA	ubiquitination	[1, 2, 3, 4, 5, 6, 8]
187	WELLMNVKRGTYLPQ	ubiquitination	[3, 6, 8]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C- terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation (By similarity).

DOMAIN 136 230 BRICHOS.
MOD_RES 37 37 Phosphothreonine (By similarity).
CARBOHYD 169 169 N-linked (GlcNAc...) (Probable).

Alternative splicing; Cell membrane; Cleavage on pair of basic residues; Complete proteome; Glycoprotein; Lysosome; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005794; C:Golgi apparatus; IDA:LIFEdb.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
GO:0005764; C:lysosome; IDA:UniProtKB.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:Compara.
GO:0001540; F:beta-amyloid binding; IPI:BHF-UCL.
GO:0012502; P:induction of programmed cell death; IEA:Compara.
GO:0010977; P:negative regulation of neuron projection development; IDA:UniProtKB.
GO:0030182; P:neuron differentiation; IDA:UniProtKB.

IPR007084; BRICHOS_dom.