UniProt Accession

 PUF60_MOUSE; Q3UEB3; Q3TGC6; Q91VR6 

Genbank Protein ID

 AK149632; AK151526; AK168789; BC010601 

Genbank Nucleotide ID

 BAE28998.1; BAE30474.1; BAE40622.1; AAH10601.1 

Protein Name

 Poly(U)-binding-splicing factor PUF60 

Protein Synonyms/Alias

 60 kDa poly(U)-binding-splicing factor 

Gene Name

 Puf60 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
85	YAMEQSIKSVLVKQT	acetylation	[1]
85	YAMEQSIKSVLVKQT	ubiquitination	[2]
248	DLSDDDIKSVFEAFG	ubiquitination	[2]
256	SVFEAFGKIKSCTLA	acetylation	[1]
256	SVFEAFGKIKSCTLA	ubiquitination	[2]
272	DPTTGKHKGYGFIEY	ubiquitination	[2]
306	GQYLRVGKAVTPPMP	ubiquitination	[2]
459	ARHMVMQKLLRKQES	acetylation	[1, 3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'- splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity). 

Sequence Annotation

 DOMAIN 134 212 RRM 1.
 DOMAIN 231 309 RRM 2.
 DOMAIN 467 554 RRM 3; atypical.
 REGION 1 521 Inhibits homodimerization (By
 REGION 82 564 Inhibits transcriptional repression,
 MOD_RES 65 65 Phosphothreonine (By similarity).
 MOD_RES 117 117 Phosphoserine (By similarity).
 MOD_RES 256 256 N6-acetyllysine (By similarity).
 MOD_RES 319 319 Phosphothreonine (By similarity).
 MOD_RES 459 459 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Apoptosis; Complete proteome; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 564 AA 

Protein Sequence

Gene Ontology

 GO:0019907; C:cyclin-dependent protein kinase activating kinase holoenzyme complex; IEA:Compara.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006532; PolyU-bd.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS