CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004178
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycogen [starch] synthase, muscle 
Protein Synonyms/Alias
  
Gene Name
 GYS1 
Gene Synonyms/Alias
 GYS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50IYTVLQTKAKVTGDEubiquitination[1, 2, 3]
52TVLQTKAKVTGDEWGubiquitination[1, 2, 3, 4]
88EAPTPALKRTLDSMNubiquitination[1, 2, 3, 4, 5, 6]
97TLDSMNSKGCKVYFGubiquitination[3, 4]
231LENFNVDKEAGERQIubiquitination[3]
271IEAQHLLKRKPDIVTubiquitination[3]
273AQHLLKRKPDIVTPNubiquitination[3]
286PNGLNVKKFSAMHEFubiquitination[3]
301QNLHAQSKARIQEFVubiquitination[3]
381NFNVETLKGQAVRKQubiquitination[1, 3, 6, 7, 8]
387LKGQAVRKQLWDTANubiquitination[3]
397WDTANTVKEKFGRKLubiquitination[1, 3]
399TANTVKEKFGRKLYEubiquitination[3]
403VKEKFGRKLYESLLVubiquitination[3]
422DMNKMLDKEDFTMMKubiquitination[3]
429KEDFTMMKRAIFATQubiquitination[3, 6]
599LSDLLDWKYLGRYYMubiquitination[1, 3, 5, 6, 7, 8, 9]
615ARHMALSKAFPEHFTubiquitination[1, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Transfers the glycosyl residue from UDP-Glc to the non- reducing end of alpha-1,4-glucan. 
Sequence Annotation
 BINDING 39 39 UDP-glucose (By similarity).
 MOD_RES 8 8 Phosphoserine; by AMPK and PKA (By
 MOD_RES 11 11 Phosphoserine (By similarity).
 MOD_RES 641 641 Phosphoserine.
 MOD_RES 645 645 Phosphoserine.
 MOD_RES 649 649 Phosphoserine.
 MOD_RES 653 653 Phosphoserine; by GSK3-alpha and GSK3-
 MOD_RES 657 657 Phosphoserine; by CK2 (By similarity).
 MOD_RES 698 698 Phosphoserine (By similarity).
 MOD_RES 727 727 Phosphoserine.  
Keyword
 Allosteric enzyme; Alternative splicing; Complete proteome; Diabetes mellitus; Disease mutation; Glycogen biosynthesis; Glycosyltransferase; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 737 AA 
Protein Sequence
MPLNRTLSMS SLPGLEDWED EFDLENAVLF EVAWEVANKV GGIYTVLQTK AKVTGDEWGD 60
NYFLVGPYTE QGVRTQVELL EAPTPALKRT LDSMNSKGCK VYFGRWLIEG GPLVVLLDVG 120
ASAWALERWK GELWDTCNIG VPWYDREAND AVLFGFLTTW FLGEFLAQSE EKPHVVAHFH 180
EWLAGVGLCL CRARRLPVAT IFTTHATLLG RYLCAGAVDF YNNLENFNVD KEAGERQIYH 240
RYCMERAAAH CAHVFTTVSQ ITAIEAQHLL KRKPDIVTPN GLNVKKFSAM HEFQNLHAQS 300
KARIQEFVRG HFYGHLDFNL DKTLYFFIAG RYEFSNKGAD VFLEALARLN YLLRVNGSEQ 360
TVVAFFIMPA RTNNFNVETL KGQAVRKQLW DTANTVKEKF GRKLYESLLV GSLPDMNKML 420
DKEDFTMMKR AIFATQRQSF PPVCTHNMLD DSSDPILTTI RRIGLFNSSA DRVKVIFHPE 480
FLSSTSPLLP VDYEEFVRGC HLGVFPSYYE PWGYTPAECT VMGIPSISTN LSGFGCFMEE 540
HIADPSAYGI YILDRRFRSL DDSCSQLTSF LYSFCQQSRR QRIIQRNRTE RLSDLLDWKY 600
LGRYYMSARH MALSKAFPEH FTYEPNEADA AQGYRYPRPA SVPPSPSLSR HSSPHQSEDE 660
EDPRNGPLEE DGERYDEDEE AAKDRRNIRA PEWPRRASCT SSTSGSKRNS VDTATSSSLS 720
TPSEPLSPTS SLGEERN 737 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016234; C:inclusion body; IEA:Compara.
 GO:0005536; F:glucose binding; IEA:Compara.
 GO:0004373; F:glycogen (starch) synthase activity; IDA:UniProtKB.
 GO:0006006; P:glucose metabolic process; TAS:Reactome.
 GO:0005978; P:glycogen biosynthetic process; IDA:UniProtKB.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR008631; Glycogen_synth. 
Pfam
 PF05693; Glycogen_syn 
SMART
  
PROSITE
  
PRINTS