UniProt Accession

 BZLF1_EBVB9; P03206; Q69127; Q777E5 

Genbank Protein ID

 V01555; AJ507799; M17547 

Genbank Nucleotide ID

 CAA24861.1; CAD53423.1; AAA66529.1 

Protein Name

 Trans-activator protein BZLF1 

Protein Synonyms/Alias

 EB1; Zebra 

Gene Name

 BZLF1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) 

NCBI Taxa ID

 10377 

Lysine Modification

Position	Peptide	Type	References
12	NSTSEDVKFTPDPYQ	sumoylation	[1, 2, 3, 4]

Reference

 [1] Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies.
 Adamson AL, Kenney S.
 J Virol. 2001 Mar;75(5):2388-99. [PMID: 11160742]
 [2] Effects of SUMO-1 upon Epstein-Barr virus BZLF1 function and BMRF1 expression.
 Adamson AL.
 Biochem Biophys Res Commun. 2005 Oct 14;336(1):22-8. [PMID: 16112644]
 [3] Sumoylation of the Epstein-Barr virus BZLF1 protein inhibits its transcriptional activity and is regulated by the virus-encoded protein kinase.
 Hagemeier SR, Dickerson SJ, Meng Q, Yu X, Mertz JE, Kenney SC.
 J Virol. 2010 May;84(9):4383-94. [PMID: 20181712]
 [4] Transcriptional repression by sumoylation of Epstein-Barr virus BZLF1 protein correlates with association of histone deacetylase.
 Murata T, Hotta N, Toyama S, Nakayama S, Chiba S, Isomura H, Ohshima T, Kanda T, Tsurumi T.
 J Biol Chem. 2010 Jul 30;285(31):23925-35. [PMID: 20516063] 

Functional Description

 Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1). 

Sequence Annotation

 DOMAIN 170 228 bZIP.
 REGION 178 195 Basic motif (By similarity).
 REGION 196 228 Leucine-zipper (By similarity).  

Keyword

 3D-structure; Complete proteome; DNA-binding; Early protein; G0/G1 host cell cycle checkpoint dysregulation by virus; Host nucleus; Host-virus interaction; Inhibition of host IFN-mediated response initiation by virus; Inhibition of host innate immune response by virus; Inhibition of host IRF7 by virus; Modulation of host cell cycle by virus; Reference proteome; Transcription; Transcription regulation; Viral immunoevasion. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 245 AA 

Protein Sequence

Gene Ontology

 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
 GO:0039557; P:suppression by virus of host IRF7 activity; IEA:UniProtKB-KW.
 GO:0039501; P:suppression by virus of host type I interferon production; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004827; bZIP.
 IPR017339; Trans-activator_BZLF1_HHV-4. 

Pfam

 PF00170; bZIP_1 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS