CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Forkhead box protein O4 
Protein Synonyms/Alias
 Fork head domain transcription factor AFX1 
Gene Name
 FOXO4 
Gene Synonyms/Alias
 AFX; AFX1; MLLT7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
186MLNPEGGKSGKAPRRacetylation[1]
189PEGGKSGKAPRRRAAacetylation[1]
407GGLPSSSKLATGVGLacetylation[1]
Reference
 [1] Negative regulation of forkhead transcription factor AFX (Foxo4) by CBP-induced acetylation.
 Fukuoka M, Daitoku H, Hatta M, Matsuzaki H, Umemura S, Fukamizu A.
 Int J Mol Med. 2003 Oct;12(4):503-8. [PMID: 12964026] 
Functional Description
 Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. 
Sequence Annotation
 DNA_BIND 100 188 Fork-head.
 MOD_RES 32 32 Phosphothreonine; by PKB/AKT1.
 MOD_RES 197 197 Phosphoserine; by PKB/AKT1.
 MOD_RES 200 200 Phosphoserine.
 MOD_RES 262 262 Phosphoserine; by PKB/AKT1.  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Cell cycle; Chromosomal rearrangement; Complete proteome; Cytoplasm; Developmental protein; Differentiation; DNA-binding; Myogenesis; Nucleus; Pharmaceutical; Phosphoprotein; Proto-oncogene; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MDPGNENSAT EAAAIIDLDP DFEPQSRPRS CTWPLPRPEI ANQPSEPPEV EPDLGEKVHT 60
EGRSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA WGNQSYAELI SQAIESAPEK 120
RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW KNSIRHNLSL HSKFIKVHNE ATGKSSWWML 180
NPEGGKSGKA PRRRAASMDS SSKLLRGRSK APKKKPSVLP APPEGATPTS PVGHFAKWSG 240
SPCSRNREEA DMWTTFRPRS SSNASSVSTR LSPLRPESEV LAEEIPASVS SYAGGVPPTL 300
NEGLELLDGL NLTSSHSLLS RSGLSGFSLQ HPGVTGPLHT YSSSLFSPAE GPLSAGEGCF 360
SSSQALEALL TSDTPPPPAD VLMTQVDPIL SQAPTLLLLG GLPSSSKLAT GVGLCPKPLE 420
APGPSSLVPT LSMIAPPPVM ASAPIPKALG TPVLTPPTEA ASQDRMPQDL DLDMYMENLE 480
CDMDNIISDL MDEGEGLDFN FEPDP 505 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0008301; F:DNA binding, bending; IBA:RefGenome.
 GO:0003690; F:double-stranded DNA binding; IBA:RefGenome.
 GO:0019901; F:protein kinase binding; IBA:RefGenome.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IMP:UniProtKB.
 GO:0007050; P:cell cycle arrest; IDA:UniProtKB.
 GO:0009790; P:embryo development; IBA:RefGenome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0000080; P:G1 phase of mitotic cell cycle; IDA:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; IDA:UniProtKB.
 GO:0007095; P:mitotic G2 DNA damage checkpoint; IBA:RefGenome.
 GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
 GO:0016525; P:negative regulation of angiogenesis; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0051151; P:negative regulation of smooth muscle cell differentiation; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0048513; P:organ development; IBA:RefGenome.
 GO:0007389; P:pattern specification process; IBA:RefGenome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IBA:RefGenome.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0048863; P:stem cell differentiation; IMP:UniProtKB.
 GO:0009888; P:tissue development; IBA:RefGenome. 
Interpro
 IPR001766; TF_fork_head.
 IPR018122; TF_fork_head_CS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00250; Fork_head 
SMART
 SM00339; FH 
PROSITE
 PS00657; FORK_HEAD_1
 PS00658; FORK_HEAD_2
 PS50039; FORK_HEAD_3 
PRINTS
 PR00053; FORKHEAD.