CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012641
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-conjugating enzyme E2 variant 2 
Protein Synonyms/Alias
 DDVit 1; Enterocyte differentiation-associated factor 1; EDAF-1; Enterocyte differentiation-promoting factor 1; EDPF-1; MMS2 homolog; Vitamin D3-inducible protein 
Gene Name
 UBE2V2 
Gene Synonyms/Alias
 MMS2; UEV2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAVSTGVKVPRNFRLubiquitination[1, 2]
66ENRIYSLKVECGPKYubiquitination[1, 2, 3, 4]
72LKVECGPKYPEAPPSacetylation[5]
72LKVECGPKYPEAPPSubiquitination[2, 3]
108RSIPVLAKWQNSYSIubiquitination[2, 3]
129LRRLMMSKENMKLPQubiquitination[1]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. 
Sequence Annotation
  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 145 AA 
Protein Sequence
MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI IGPPRTNYEN 60
RIYSLKVECG PKYPEAPPSV RFVTKINMNG INNSSGMVDA RSIPVLAKWQ NSYSIKVVLQ 120
ELRRLMMSKE NMKLPQPPEG QTYNN 145 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:HGNC.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0031372; C:UBC13-MMS2 complex; IDA:HGNC.
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0000729; P:DNA double-strand break processing; IMP:HGNC.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0045739; P:positive regulation of DNA repair; IEA:Compara.
 GO:0010976; P:positive regulation of neuron projection development; IEA:Compara.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0051965; P:positive regulation of synapse assembly; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; TAS:ProtInc.
 GO:0006282; P:regulation of DNA repair; TAS:ProtInc. 
Interpro
 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF00179; UQ_con 
SMART
  
PROSITE
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS