UniProt Accession

 FZR_HUMAN; Q9UM11; O75869; Q86U66; Q96NW8; Q9UI96; Q9ULH8; Q9UM10; Q9UNQ1; Q9Y2T8 

Genbank Protein ID

 AF102508; AF102507; AB013462; AB013463; AF080397; AF083810; AF433157; BT007115; AC005787; AC005786; AB033068; BC013413 

Genbank Nucleotide ID

 AAD26624.1; AAD26623.1; BAA86954.1; BAA86955.1; AAF20266.1; AAD52030.1; AAL28117.1; AAP35779.1; AAC62835.1; AAC62836.1; BAA86556.1; AAH13413.1 

Protein Name

 Fizzy-related protein homolog 

Protein Synonyms/Alias

 Fzr; CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1 

Gene Name

 FZR1 

Gene Synonyms/Alias

 CDH1; FYR; FZR; KIAA1242 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
86	DATSDNGKDGLAYSA	ubiquitination	[1]
96	LAYSALLKNELLGAG	ubiquitination	[1]
106	LLGAGIEKVQDPQTE	ubiquitination	[1]
125	QPSTPEKKGLFTYSL	ubiquitination	[1]
135	FTYSLSTKRSSPDDG	ubiquitination	[1]
156	SLSPVSNKSQKLLRS	ubiquitination	[1]
173	KPTRKISKIPFKVLD	ubiquitination	[1]
262	IWDAAAGKKLSMLEG	ubiquitination	[1, 2]
263	WDAAAGKKLSMLEGH	ubiquitination	[1]
320	RQEVCGLKWSTDHQL	ubiquitination	[1]
336	ASGGNDNKLLVWNHS	ubiquitination	[1]
429	QNQILVWKYPSLTQV	ubiquitination	[1]
475	RFWNVFSKTRSTKES	ubiquitination	[1]
480	FSKTRSTKESVSVLN	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. 

Sequence Annotation

 REPEAT 182 222 WD 1.
 REPEAT 227 266 WD 2.
 REPEAT 269 306 WD 3.
 REPEAT 311 350 WD 4.
 REPEAT 353 395 WD 5.
 REPEAT 397 438 WD 6.
 REPEAT 441 480 WD 7.
 MOD_RES 32 32 Phosphothreonine.
 MOD_RES 36 36 Phosphoserine.
 MOD_RES 138 138 Phosphoserine.
 MOD_RES 146 146 Phosphoserine.
 MOD_RES 151 151 Phosphoserine.  

Keyword

 Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; DNA damage; DNA repair; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 496 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0051488; P:activation of anaphase-promoting complex activity; IDA:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0031572; P:G2 DNA damage checkpoint; IDA:UniProtKB.
 GO:0070306; P:lens fiber cell differentiation; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0090344; P:negative regulation of cell aging; IEA:Compara.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0045732; P:positive regulation of protein catabolic process; IGI:UniProtKB.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0070979; P:protein K11-linked ubiquitination; TAS:UniProtKB.
 GO:0040020; P:regulation of meiosis; IEA:Compara. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS