CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017394
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/WD repeat-containing protein 8 
Protein Synonyms/Alias
 F-box and WD-40 domain-containing protein 8; F-box only protein 29 
Gene Name
 FBXW8 
Gene Synonyms/Alias
 FBW6; FBW8; FBX29; FBXO29; FBXW6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
427EAGRRLLKLGNVLRDubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Substrate-recognition component of a SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. In complex with CUL7, mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. 
Sequence Annotation
 DOMAIN 113 159 F-box.
 REPEAT 197 245 WD 1.
 REPEAT 254 294 WD 2.
 REPEAT 297 336 WD 3.
 REPEAT 430 470 WD 4.
 REPEAT 473 510 WD 5.
 MOD_RES 85 85 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Golgi apparatus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 598 AA 
Protein Sequence
MDDYSLDEFR RRWQEELAQA QAPKKRRRPE AAERRARRPE VGSGRGEQAS GDPALAQRLL 60
EGAGRPPAAR ATRAEGQDVA SRSRSPLARE GAGGGEQLVD QLIRDLNEMN DVPFFDIQLP 120
YELAINIFQY LDRKELGRCA QVSKTWKVIA EDEVLWYRLC QQEGHLPDSS ISDYSCWKLI 180
FQECRAKEHM LRTNWKNRKG AVSELEHVPD TVLCDVHSHD GVVIAGYTSG DVRVWDTRTW 240
DYVAPFLESE DEEDEPGMQP NVSFVRINSS LAVAAYEDGF LNIWDLRTGK YPVHRFEHDA 300
RIQALALSQD DATVATASAF DVVMLSPNEE GYWQIAAEFE VPKLVQYLEI VPETRRYPVA 360
VAAAGDLMYL LKAEDSARTL LYAHGPPVTC LDVSANQVAF GVQGLGWVYE GSKILVYSLE 420
AGRRLLKLGN VLRDFTCVNL SDSPPNLMVS GNMDGRVRIH DLRSGNIALS LSAHQLRVSA 480
VQMDDWKIVS GGEEGLVSVW DYRMNQKLWE VYSGHPVQHI SFSSHSLITA NVPYQTVMRN 540
ADLDSFTTHR RHRGLIRAYE FAVDQLAFQS PLPVCRSSCD AMATHYYDLA LAFPYNHV 598 
Gene Ontology
 GO:0031467; C:Cul7-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0019005; C:SCF ubiquitin ligase complex; IEA:Compara.
 GO:0060716; P:labyrinthine layer blood vessel development; IEA:Compara.
 GO:0060712; P:spongiotrophoblast layer development; IEA:Compara. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00646; F-box
 PF00400; WD40 
SMART
 SM00256; FBOX
 SM00320; WD40 
PROSITE
 PS50181; FBOX
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS