UniProt Accession

 PCKGC_HUMAN; P35558; A8K437; Q8TCA3; Q9UJD2 

Genbank Protein ID

 L05144; L12760; AY794987; AK290802; AL035541; CH471077; BC023978; U31519 

Genbank Nucleotide ID

 AAA60084.1; AAA02558.1; AAV50001.1; BAF83491.1; CAB55863.1; EAW75514.1; AAH23978.1; AAA91026.1 

Protein Name

 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] 

Protein Synonyms/Alias

 PEPCK-C; Phosphoenolpyruvate carboxylase 

Gene Name

 PCK1 

Gene Synonyms/Alias

 PEPCK1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
70	EGILRRLKKYDNCWL	acetylation	[1, 2]
71	GILRRLKKYDNCWLA	acetylation	[1, 2]
243	GGNSLLGKKCFALRM	ubiquitination	[3]
471	ATAAAEHKGKIIMHD	ubiquitination	[4]
594	KEVEDIEKYLEDQVN	acetylation	[1, 2]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Acetylation regulates gluconeogenesis by promoting PEPCK1 degradation via recruiting the UBR5 ubiquitin ligase.
 Jiang W, Wang S, Xiao M, Lin Y, Zhou L, Lei Q, Xiong Y, Guan KL, Zhao S.
 Mol Cell. 2011 Jul 8;43(1):33-44. [PMID: 21726808]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. 

Sequence Annotation

 NP_BIND 287 292 GTP.
 NP_BIND 530 533 GTP.
 REGION 403 405 Substrate binding.
 ACT_SITE 288 288
 METAL 244 244 Manganese.
 METAL 264 264 Manganese.
 METAL 311 311 Manganese.
 BINDING 87 87 Substrate.
 BINDING 237 237 Substrate; via amide nitrogen.
 BINDING 244 244 Substrate (By similarity).
 BINDING 286 286 Substrate (By similarity).
 BINDING 405 405 GTP.
 BINDING 436 436 GTP.
 MOD_RES 70 70 N6-acetyllysine; by p300/EP300.
 MOD_RES 71 71 N6-acetyllysine; by p300/EP300.
 MOD_RES 594 594 N6-acetyllysine; by p300/EP300.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding; Polymorphism; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 622 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031406; F:carboxylic acid binding; IDA:BHF-UCL.
 GO:0019003; F:GDP binding; IEA:Compara.
 GO:0005525; F:GTP binding; IDA:BHF-UCL.
 GO:0000287; F:magnesium ion binding; IDA:BHF-UCL.
 GO:0030145; F:manganese ion binding; IDA:BHF-UCL.
 GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; ISS:BHF-UCL.
 GO:0017144; P:drug metabolic process; TAS:Reactome.
 GO:0006094; P:gluconeogenesis; ISS:BHF-UCL.
 GO:0042593; P:glucose homeostasis; ISS:BHF-UCL.
 GO:0046327; P:glycerol biosynthetic process from pyruvate; ISS:BHF-UCL.
 GO:0006475; P:internal protein amino acid acetylation; IDA:UniProtKB.
 GO:0006107; P:oxaloacetate metabolic process; IEA:Compara.
 GO:0014823; P:response to activity; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IDA:BHF-UCL. 

Interpro

 IPR018091; PEP_carboxykin_GTP_CS.
 IPR013035; PEP_carboxykinase_C.
 IPR008209; PEP_carboxykinase_GTP.
 IPR008210; PEP_carboxykinase_N. 

Pfam

 PF00821; PEPCK 

SMART

  

PROSITE

 PS00505; PEPCK_GTP 

PRINTS