CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018100
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase haspin 
Protein Synonyms/Alias
 Germ cell-specific gene 2 protein; H-haspin; Haploid germ cell-specific nuclear protein kinase 
Gene Name
 GSG2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
229EEATGGAKDTRMVHQubiquitination[1, 2]
274RESCCKRKLVVGNGPubiquitination[1]
291PGLSSTGKRRATGQDubiquitination[1, 2]
456TLSISNKKASDAEKVubiquitination[1]
462KKASDAEKVYGECSQubiquitination[1]
470VYGECSQKGPVPFSHubiquitination[2]
483SHCLPTEKLQRCEKIubiquitination[1]
618DLEQMRTKLSSLATAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. 
Sequence Annotation
 DOMAIN 484 798 Protein kinase.
 NP_BIND 490 498 ATP (By similarity).
 NP_BIND 606 611 ATP.
 NP_BIND 649 654 ATP.
 NP_BIND 687 689 ATP.
 ACT_SITE 649 649 Proton acceptor (By similarity).
 BINDING 511 511 ATP.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 93 93 Phosphoserine; by AURKB.
 MOD_RES 97 97 Phosphothreonine.
 MOD_RES 143 143 Phosphoserine; by AURKB.
 MOD_RES 147 147 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Chromatin regulator; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 798 AA 
Protein Sequence
MAASLPGPGS RLFRTYGAAD GRRQRRPGRE AAQWFPPQDR RRFFNSSGSS DASIGDPSQS 60
DDPDDPDDPD FPGSPVRRRR RRPGGRVPKD RPSLTVTPKR WKLRARPSLT VTPRRLGLRA 120
RPPQKCSTPC GPLRLPPFPS RDSGRLSPDL SVCGQPRDGD ELGISASLFS SLASPCPGSP 180
TPRDSVISIG TSACLVAASA VPSGLHLPEV SLDRASLPCS QEEATGGAKD TRMVHQTRAS 240
LRSVLFGLMN SGTPEDSEFR ADGKNMRESC CKRKLVVGNG PEGPGLSSTG KRRATGQDSC 300
QERGLQEAVR REHQEASVPK GRIVPRGIDR LERTRSSRKS KHQEATETSL LHSHRFKKGQ 360
KLGKDSFPTQ DLTPLQNVCF WTKTRASFSF HKKKIVTDVS EVCSIYTTAT SLSGSLLSEC 420
SNRPVMNRTS GAPSSWHSSS MYLLSPLNTL SISNKKASDA EKVYGECSQK GPVPFSHCLP 480
TEKLQRCEKI GEGVFGEVFQ TIADHTPVAI KIIAIEGPDL VNGSHQKTFE EILPEIIISK 540
ELSLLSGEVC NRTEGFIGLN SVHCVQGSYP PLLLKAWDHY NSTKGSANDR PDFFKDDQLF 600
IVLEFEFGGI DLEQMRTKLS SLATAKSILH QLTASLAVAE ASLRFEHRDL HWGNVLLKKT 660
SLKKLHYTLN GKSSTIPSCG LQVSIIDYTL SRLERDGIVV FCDVSMDEDL FTGDGDYQFD 720
IYRLMKKENN NRWGEYHPYS NVLWLHYLTD KMLKQMTFKT KCNTPAMKQI KRKIQEFHRT 780
MLNFSSATDL LCQHSLFK 798 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0072354; F:histone kinase activity (H3-T3 specific); IMP:UniProtKB.
 GO:2000751; P:histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore; IMP:UniProtKB.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0007064; P:mitotic sister chromatid cohesion; IMP:UniProtKB.
 GO:0071459; P:protein localization to chromosome, centromeric region; IMP:UniProtKB.
 GO:0090231; P:regulation of spindle checkpoint; IMP:UniProtKB. 
Interpro
 IPR024604; DUF3635.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS. 
Pfam
 PF12330; DUF3635
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS